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It is a sequence of DNA that is also a palindrom. i.e. the complimentary sequence of DNA would read the same way (but in the other direction). g a a t t c
c t t a a g Moreover it is the sequence of DNA recognised by the restriction endonuclease EcoR1, the first such enzyme to be discovered. These enzymes have been important tools in science allowing pieces of DNA to be specifically excised and manipulated.
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Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What sticky ends does EcoR1 create?
EcoR1 creates sticky ends with a sequence of 5'-GAATTC-3'. This results in protruding ends with a 5' overhang on both strands of the DNA.
Explain the Molecular mechanism functions of restriction endonucleases?
Restriction Endonucleases recognize certain sites on the DNA or the sequences. For example EcoR1 that recognizes the restriction site GAATTC on any strand of DNA or RNA.
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
5 example of palindromic DNA sequences?
Palindromic DNA sequences are segments of DNA that read the same forwards and backwards on complementary strands. Five examples include: 1) EcoRI recognition site: GAATTC, 2) HindIII recognition site: AAGCTT, 3) BamHI recognition site: GGATCC, 4) NotI recognition site: GCGGCCGC, and 5) NheI recognition site: GCTAGC. These sequences are often the target sites for restriction enzymes in molecular biology.
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
How do biologists make use of restriction enzymes?
They cut strands of DNA at specific sites.
What does the formation of recombinant DNA result from?
Recombinant DNATo to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'3' CTTAAG 5'Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:5' G|AATTC 3'3' CTTAA|G 5'Then the strands would separate:5' G--------AATTC 3'3' CTTAA--------G 5'Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.
