Its the command center of the cell.
Because damaged mitochondria will not exhibit activity. Succinate dehydrogenase is the best option. Refer pg 32 subcellular fractionation- a practical approach.
Most summaries of the Krebs Cycle will usually indicate that the cycle is an aerobic process (one that requires oxygen) that produces ATP by breaking down glucose.Kreb Cycle shows no oxygen or glucose is used in the cycle and that it does not make much ATP (only one molecule for each acetyl CoA that enters the cycle).
mostly it is found in mitochondria as it is used in the TCA cycle
ADH (alcohol dehydrogenase) ADH (alcohol dehydrogenase)
In outer membrane:- Monoamine oxidase, Fatty acid thiokinase, Kynurenine hydroxylase In pereimitochondrial space:- Adenylate kinase, Nucleoside diphosphokinase In inner membrane:- Succinate dehydrogenase, alpha Keto acid dehydrogenase
Malonic acid is a competitive inhibitor of succinate dehydrogenase.
Malonate is a competitive inhibitor of succinate dehydrogenase, the enzyme responsible for converting succinate to fumarate in the citric acid cycle. Malonate resembles succinate and competes for the active site of succinate dehydrogenase. As a result, malonate binds to the enzyme and prevents succinate from binding, inhibiting the conversion of succinate to fumarate.
All through the Citric Acid Cycle. Succinate dehydrogenase oxidizes succinate.
includes a succinate dehydrogenase
alpha-ketoglutarate dehydrogenase is most similar to pyruvate dehydrogenase as both are enzyme complexes made of 3 units
Complex 2 (succinate dehydrogenase)
Because damaged mitochondria will not exhibit activity. Succinate dehydrogenase is the best option. Refer pg 32 subcellular fractionation- a practical approach.
Some enzymes are present in certain organelles only; such specific enzymes are called as marker enzymes. After centrifugation, the separated organelles are identified by detection of marker enzymes in the sample. Mitochondria=> Inner membrane: ATP Synthase. Lysosome=> Cathepsin. Golgi complex=> Galactosyl transferase. Microsomes=> Glucose-6-phosphate. Cytoplasm=> Lactate dehydrogenase.
There are very many enzymes involved. A few from glycolysis are: hexokinase and glucokinase, phosphohexose isomerase, phosphofructokinase, pyruvate kinase; from pyruvate decarboxylation are pyruvate dehydrogenase phosphatase and pyruvate dehydrogenase kinase; and a few from the Kreb's cycle are: aconitase, alpha-ketoglutamate dehydrogenase, succinate thiokinase, and fumarase.
Most summaries of the Krebs Cycle will usually indicate that the cycle is an aerobic process (one that requires oxygen) that produces ATP by breaking down glucose.Kreb Cycle shows no oxygen or glucose is used in the cycle and that it does not make much ATP (only one molecule for each acetyl CoA that enters the cycle).
Correct answer: II and III
in cyptoplasm of the prokaryotic cell