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A primer of RNA
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∙ 12y ago
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For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
RNA Primer
Why RNA primers are needed during DNA replication and what is their fate?
DNA polymerase cannot begin the synthesis of new DNA.To synthesis a new strand of DNA ,RNA primer is required.The complementary RNA nucleotides,that are added opposite to the single strand of parent DNA are the RNA primer.
Why can't DNA polymerase synthesize a new strand of DNA without the help of a RNA primer?
Because DNA Polymerase requires the OH on the 3' as an active site. It uses the OH on the 3' end of a nucleotide to attach a phosphate from the 5' end of the next nucleotide. It only works in this direction, and that is why DNA polymerase works 5' to 3'.
How can DNA be prepared for visualization?
The DNA can be denatured first then follow the annealing process by any primer and allow the extension phase to follow it and remember in this experiment the first step requires a heat resistant DNA polymerase and then do the electrophoresis and then you put the sample under the transilluminator and observe
What are primer dimers in pcr?
Primerdimer occur, when the Primer are -or parts- are complementary (3' of the FOR- and 3' of the REV-Primer). While PCR both oligos hybridizate and are elongated. The Product contains both primer sequences. Primerdimers reduce the avaiable ammount of primers for the pcr-reaction. Therefore the pcr effectivity is reduced because of this non-specific reaction.
Nucleotide sequence that starts PCR processes?
Primer sequences
What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?
An RNA primer.
For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
RNA Primer
What is the force needed to to fire a 9mm bullet?
A primer will usually get it out of the case.
Is 34 primer?
34 is not a prime number. In addition to '1' and itself, it has two more factors: 2 and 17 .
For DNA polymerase to link nucleotides together as new strands of DNA the first nucleotide must be attached to what because DNA polymerase can only add nucleotides to a pre exsisting strand?
RNA Primer
How much is the momentum needed to fire a 9mm bullet?
Usually just a primer will get it partway down the barrel.
Do paints come with primer in them?
No, you buy the primer separate and paint with it first, let it dry then get the paint and paint over the primer. There are several paint applications where the paint can be used as a primer, and this is called a self-priming paint. In addition, many paints are marketed as a paint and primer in one, however, that refers to its ability to adhere well to a surface and to cover the previous coat. In more difficult priming situations, like staining or painting with a very dark color over a light color, it's recommended to use a dedicated primer followed by a top coat.
What are the enzymes involved in replication?
There are a variety of enzymes used in replication. Helicase is used to open the hydrogen bonds that connect the two strands. However, this causes a tension to form in the strands (like a wind up toy) so some of it needs to be released. This is done by topoisomerase, which cuts the strands, lets them spin out some of the tension and attaches the DNA back together again. Moving behind helicase, is an enzyme called SSBP. This basically binds to the DNA sequence to prevent it from reattaching to itself after helicase unzips it; DNA would otherwise just bond back with the other strand. Then an RNA Polymerase called primase comes and attaches a primer to the DNA strands. This is needed because the next enzyme, DNA polymerase will not from scratch and needs a base to work from: the primer serves this role. Starting on the primer, DNA Polymerase III synthesizes the new strand, but the primers are still left on the strands. These will be removed by DNA Polymerase I which also adds new nucleotides to the hole left by the primer. Finally, an enzyme called ligase fills the one nucleotide gap left between the primer and the newly synthesized DNA with a sugar phosphate backbone (not another nucleotide)
How much paint base coat clear coat primer and color is needed by an OEM for a new standard vehicle?
balls
Can you use the aloe plant to make face primer or can i use extreme gel that's the name of the gel?
Yes, a person can use the aloe plant to make face primer or they can use extreme gel. Some of the things needed to make face primer besides the aloe plant are Vitamin C and Vitamin E.
What is two major rules of DNA polymerase in the process of DNA replication?
One major rule of DNA polymerase is that it can only synthesize DNA in the 5' to 3' direction. This means that it adds nucleotides to the growing DNA strand by linking the 3' end of the incoming nucleotide with the 5' phosphate group of the previous nucleotide. Another rule is that DNA polymerase requires a primer, a short segment of RNA or DNA, to initiate DNA synthesis. The primer provides a starting point for DNA polymerase to begin adding nucleotides.
