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What is the enzyme that synthesized DNA used in PCR that distinguishes it from the equivalent enzyme that carry out the same function in our cells or those of most bacteria?
The enzyme used in PCR to synthesize DNA is called DNA polymerase. The key difference is that the DNA polymerase used in PCR, such as Taq polymerase, is derived from a thermophilic bacterium called Thermus aquaticus and can withstand the high temperatures used in the PCR cycling process. This distinguishes it from the equivalent enzyme in our cells or most bacteria, which would be denatured by the high temperatures of PCR.
What is the recommended extension time for Taq polymerase in PCR amplification?
The recommended extension time for Taq polymerase in PCR amplification is typically 1 minute per kilobase of DNA being amplified.
What is the recommended extension time for the Taq polymerase in PCR reactions?
The recommended extension time for Taq polymerase in PCR reactions is typically 1 minute per kilobase of DNA being amplified.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What are the benefits of using Taq polymerase in PCR?
Taq polymerase is beneficial in PCR because it is heat-resistant, allowing for the high temperatures needed to separate DNA strands. This enzyme also has a high replication rate, leading to faster PCR cycles. Additionally, Taq polymerase is cost-effective and widely available, making it a popular choice for PCR experiments.
Role of Taq polymerase in PCR?
taq polymerase is special because it is very stable at high temperatures and will not denature even at the 90 degree step of pcr. taq polymerase is so heat stable because it was extracted from the bacterium thermus aquaticus, which is found in hot springs and geezers
What is the enzyme that synthesized DNA used in PCR that distinguishes it from the equivalent enzyme that carry out the same function in our cells or those of most bacteria?
The enzyme used in PCR to synthesize DNA is called DNA polymerase. The key difference is that the DNA polymerase used in PCR, such as Taq polymerase, is derived from a thermophilic bacterium called Thermus aquaticus and can withstand the high temperatures used in the PCR cycling process. This distinguishes it from the equivalent enzyme in our cells or most bacteria, which would be denatured by the high temperatures of PCR.
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
What is the recommended extension time for Taq polymerase in PCR amplification?
The recommended extension time for Taq polymerase in PCR amplification is typically 1 minute per kilobase of DNA being amplified.
What is the recommended extension time for the Taq polymerase in PCR reactions?
The recommended extension time for Taq polymerase in PCR reactions is typically 1 minute per kilobase of DNA being amplified.
What organism is used primarily in PCR technique?
The organism used primarily in PCR (polymerase chain reaction) technique is a heat-stable DNA polymerase, such as Taq polymerase. Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, which can withstand the high temperatures required for PCR amplification.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What are the benefits of using Taq polymerase in PCR?
Taq polymerase is beneficial in PCR because it is heat-resistant, allowing for the high temperatures needed to separate DNA strands. This enzyme also has a high replication rate, leading to faster PCR cycles. Additionally, Taq polymerase is cost-effective and widely available, making it a popular choice for PCR experiments.
What is the source of the polymerase used in polymerase chain reaction?
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
What makes the Taq polymerase special and essential in the process of polymerase chain reaction (PCR)?
Taq polymerase is special and essential in PCR because it can withstand high temperatures needed to separate DNA strands during the reaction. This heat-resistant enzyme allows for the repeated cycles of heating and cooling required for DNA amplification, making PCR possible.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
