Chlorophyll has a function of converting starch into glucose and other simpler plant products.Thats why we have to remove cholorphyll before starting a test for starch.
I'm not sure but I think it's so that when you put the iodine on, you can see which bits have starch on.
Ethanol dissolves chlorophyll hence further phtosynthetic activity is stopped in the abscence of light and the leaf becomes transparent (colorless). the colorless leaf takes better stain with iodene while testing for the presence of starch.
A hot bath of ethanol decolorizes the leaf by washing out the chlorophyll. If the leaf is not decolorized, you cannot see the blue-black stain that results from the iodine reacting with the starch.
Starch is a complex carbohydrate. The presence of starch in leaves or plants can be detected by iodine test. In microscopic studies, starch granules can be seen under magnification inside the cells.
alcohol is used to remove chlorophyll
The starch in a variegated Coleus leaf is stored in the pigmented parts of the leaf. Coleus is a flowering plant is usually considered an ornamental plant because of its color.
Ethanol dissolves chlorophyll hence further phtosynthetic activity is stopped in the abscence of light and the leaf becomes transparent (colorless). the colorless leaf takes better stain with iodene while testing for the presence of starch.
A hot bath of ethanol decolorizes the leaf by washing out the chlorophyll. If the leaf is not decolorized, you cannot see the blue-black stain that results from the iodine reacting with the starch.
It is to remove the chloroplast and dissolve in the alcohol and turn it green.
The leaf turns brittle during the testing the leaf for starch because the ethanol extracts the all water content from the leaf.
Starch is a complex carbohydrate. The presence of starch in leaves or plants can be detected by iodine test. In microscopic studies, starch granules can be seen under magnification inside the cells.
to denature the enzymes going to kill the leaf
Use iodine to test a leaf for starch | Plant Physiology | Biology
alcohol is used to remove chlorophyll
The starch in a variegated Coleus leaf is stored in the pigmented parts of the leaf. Coleus is a flowering plant is usually considered an ornamental plant because of its color.
It is to kill the cytoplasm, denaturate the enzyme and makes the leaf become more permeable to the iodine solution, therefor, we have to boil a leaf for starch test
You can perform an experiment to show that chlorophyll is necessary by using a a potted plant having variegated leaves.(This ia a leaf having several different colours) Destarch the plant by keeping it in the dark for several days. During this time, no more starch is made and the starch present is used up in respiration. The plant is then placed in strong sulinght fo a few hours. Remove one leaf and test for starch with iodine solution. You will notice that the non-green parts of the leaf turns reddish brown due to the absence of starch. However, the green parts of the leaf turns blue black confirming the presence of starch. Hence, it is demonstrated that chlorophyll is needed in plants.
It can be observed that when testing for starch their must be a olour change of blue black after iodine solution was added.Before the colour change was green that changed to blue black of the whole procedure is been carried out.