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Enzymes involved in template directed synthesis of DNA from deoxyribonucleotide E. Coli, III appears to be most important in genome replication and I is important for its ability to edit out unpaired bases at the endstrands. Animal cells have and polymerases, with apparently responsible for replication of nuclear DNA and for replication of mitochondrial. All these function with a DNA strand as template. Retroviruses possess a unique DNA polymerase (reverse transcriptase) that uses an RNA template. triphosphates. I, II and III are known in of growing *
Yes.
DNA polymerases, like most enzymes, are proteins, and the code for their primary structure (their amino acid sequences) is included in the genome.
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Yes, DNA does code for DNA polymerase.
Every protein made by the cell (as far is known) must be coded for somewhere in the DNA. This includes proteins that fix, replicate, wind, unwind, and control gene expression of DNA. Therefore DNA holds the information needed for every protein that works with DNA and the cell.
Wiki User
∙ 11y ago
DNA polymerase requires a primer to initiate DNA synthesis because it can only add nucleotides onto an existing strand, it cannot start DNA synthesis from scratch. The primer provides a free 3'-OH group to which the polymerase can add nucleotides, allowing it to extend the DNA strand.
Wiki User
∙ 14y agoDNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.
Wiki User
∙ 10y agoNo, translation is the process of manufacturing proteins using RNA transcribed from the cell's DNA. It involves ribosomes, tRNA, and amino acids to manufacture polypeptide chains.
Wiki User
∙ 12y agoNo you just need the strand
Wiki User
∙ 13y agoyes
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What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Which of the following enzymes creates a primer for DNA polymerase?
DNA primase is the enzyme that creates the RNA primer needed for DNA polymerase to initiate DNA synthesis.
For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
For DNA polymerase to link nucleotides together, the first nucleotide must be attached to a primer, which is a short segment of RNA or DNA that provides a free 3' hydroxyl group for the DNA polymerase to start adding nucleotides. DNA polymerase can only extend nucleotides from an existing primer or strand, using it as a template for complementary base pairing.
Why rna primer is used instead of dna primer?
RNA primers are used in DNA replication because RNA primers are easier to synthesize compared to DNA primers. RNA primers are complementary to the DNA template strand and provide a starting point for DNA polymerase to begin replication. Once the new DNA strand is synthesized, the RNA primer is removed and replaced with DNA.
Why RNA primers are needed during DNA replication and what is their fate?
RNA primers are needed during DNA replication to provide a starting point for DNA polymerase to begin building a new DNA strand. Once the DNA polymerase has extended the RNA primer with DNA nucleotides, the primer is removed and replaced with DNA by DNA polymerase. The fate of RNA primers is to be replaced with DNA, ensuring that the newly synthesized DNA strand is fully composed of DNA nucleotides.
What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Which of the following enzymes creates a primer for DNA polymerase?
DNA primase is the enzyme that creates the RNA primer needed for DNA polymerase to initiate DNA synthesis.
What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?
A primer molecule is required for DNA polymerase to initiate the addition of nucleotides. This primer provides a starting point for DNA polymerase to begin adding nucleotides in the correct sequence. Once the primer is in place, DNA polymerase can add nucleotides complementary to the template strand.
Does initiation of transcription require a primer?
No, initiation of transcription does not require a primer. Transcription begins at the promoter region of a gene, where RNA polymerase binds and starts synthesizing a complementary RNA strand using the template DNA strand. In contrast, DNA replication requires a primer to initiate synthesis.
In polymerase chain reaction how many kinds of primer are used?
In polymerase chain reaction (PCR), two types of primers are used: a forward primer and a reverse primer. These short DNA sequences are specific to the target DNA region to be amplified and serve as starting points for DNA synthesis by the DNA polymerase enzyme.
For DNA polymerase to link nucleotides together as new strands of DNA the first nucleotide must be attached to what because DNA polymerase can only add nucleotides to a pre exsisting strand?
The first nucleotide must be attached to a short RNA primer to provide a free 3' hydroxyl group for DNA polymerase to extend from. DNA polymerase starts adding nucleotides to this RNA primer to begin DNA replication.
For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
For DNA polymerase to link nucleotides together, the first nucleotide must be attached to a primer, which is a short segment of RNA or DNA that provides a free 3' hydroxyl group for the DNA polymerase to start adding nucleotides. DNA polymerase can only extend nucleotides from an existing primer or strand, using it as a template for complementary base pairing.
Why rna primer is used instead of dna primer?
RNA primers are used in DNA replication because RNA primers are easier to synthesize compared to DNA primers. RNA primers are complementary to the DNA template strand and provide a starting point for DNA polymerase to begin replication. Once the new DNA strand is synthesized, the RNA primer is removed and replaced with DNA.
Why RNA primers are needed during DNA replication and what is their fate?
RNA primers are needed during DNA replication to provide a starting point for DNA polymerase to begin building a new DNA strand. Once the DNA polymerase has extended the RNA primer with DNA nucleotides, the primer is removed and replaced with DNA by DNA polymerase. The fate of RNA primers is to be replaced with DNA, ensuring that the newly synthesized DNA strand is fully composed of DNA nucleotides.
What kind of reaction is DNA replication and what does it require?
DNA replication is a semi-conservative process where a DNA molecule makes a copy of itself. It requires enzymes such as DNA polymerase, dNTPs (deoxynucleotide triphosphates), a template DNA strand, and primer to initiate the process.
What is a RNA primer removed by?
A RNA primer in DNA replication is removed by an enzyme called DNA polymerase I in prokaryotes and DNA polymerase δ in eukaryotes. These enzymes have exonuclease activity that can remove RNA primers and replace them with DNA nucleotides.
What is two major rules of DNA polymerase in the process of DNA replication?
One major rule of DNA polymerase is that it can only synthesize DNA in the 5' to 3' direction. This means that it adds nucleotides to the growing DNA strand by linking the 3' end of the incoming nucleotide with the 5' phosphate group of the previous nucleotide. Another rule is that DNA polymerase requires a primer, a short segment of RNA or DNA, to initiate DNA synthesis. The primer provides a starting point for DNA polymerase to begin adding nucleotides.
