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Enzymes involved in template directed synthesis of DNA from deoxyribonucleotide E. Coli, III appears to be most important in genome replication and I is important for its ability to edit out unpaired bases at the endstrands. Animal cells have and polymerases, with apparently responsible for replication of nuclear DNA and for replication of mitochondrial. All these function with a DNA strand as template. Retroviruses possess a unique DNA polymerase (reverse transcriptase) that uses an RNA template. triphosphates. I, II and III are known in of growing *

Yes.

DNA polymerases, like most enzymes, are proteins, and the code for their primary structure (their amino acid sequences) is included in the genome.

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Yes, DNA does code for DNA polymerase.

Every protein made by the cell (as far is known) must be coded for somewhere in the DNA. This includes proteins that fix, replicate, wind, unwind, and control gene expression of DNA. Therefore DNA holds the information needed for every protein that works with DNA and the cell.

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11y ago
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13y ago

DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.

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No, translation is the process of manufacturing proteins using RNA transcribed from the cell's DNA. It involves ribosomes, tRNA, and amino acids to manufacture polypeptide chains.

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No you just need the strand

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yes

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Q: Why does DNA polymerase requires a primer?
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What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?

DNA polymerase :)


Two major enzymes used during DNA replication?

The two main ones are DNA-polymerase and helicase


Which of the following enzymes creates a primer for DNA polymerase?

Primase


Why rna primer is used instead of dna primer?

There are basically two types of enzymes that can bind to DNA and copy it. The DNA polymerase and the RNA polymerase. The RNA polymerase, which copies DNA into RNA, will only bind to single stranded DNA, in other words areas of the DNA where the nitrogen bases holding the two strands of nucleotide units together have been separated. On the other hand the DNA polymerase that copies DNA into DNA will only bind to DNA that is double stranded. So in lies the dilemma. To make a copy of the DNA the DNA polymerase is use, but it will not bind to single stranded DNA so there is no way to make a DNA primer using aDNA polymerase, but the RNA polymerase will bind to single stranded DNA and there for can be used to make a small RNA primer on the open strands of DNA. Now the DNA polymerase has place that is double stranded and can attach and start copying the DNA.


What is the use of primer in DNA replication?

DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.

Related questions

What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?

DNA polymerase :)


Two major enzymes used during DNA replication?

The two main ones are DNA-polymerase and helicase


Which of the following enzymes creates a primer for DNA polymerase?

Primase


Why rna primer is used instead of dna primer?

There are basically two types of enzymes that can bind to DNA and copy it. The DNA polymerase and the RNA polymerase. The RNA polymerase, which copies DNA into RNA, will only bind to single stranded DNA, in other words areas of the DNA where the nitrogen bases holding the two strands of nucleotide units together have been separated. On the other hand the DNA polymerase that copies DNA into DNA will only bind to DNA that is double stranded. So in lies the dilemma. To make a copy of the DNA the DNA polymerase is use, but it will not bind to single stranded DNA so there is no way to make a DNA primer using aDNA polymerase, but the RNA polymerase will bind to single stranded DNA and there for can be used to make a small RNA primer on the open strands of DNA. Now the DNA polymerase has place that is double stranded and can attach and start copying the DNA.


What does DNA primase create?

DNA primase creates RNA primer. DNA primase is an enzyme and DNA polymerase uses the RNA primer to replicate ssDNA.


What is the use of primer in DNA replication?

DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.


What is two major rules of DNA polymerase in the process of DNA replication?

One major rule of DNA polymerase is that it can only synthesize DNA in the 5' to 3' direction. This means that it adds nucleotides to the growing DNA strand by linking the 3' end of the incoming nucleotide with the 5' phosphate group of the previous nucleotide. Another rule is that DNA polymerase requires a primer, a short segment of RNA or DNA, to initiate DNA synthesis. The primer provides a starting point for DNA polymerase to begin adding nucleotides.


Differences between DNA polymerase and RNA polymerase?

A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.


Produces The RNA primer required for DNA replication to begin?

the chemical primase produces the rna primer to start DNA replication. the primase is later removed and replaced with DNA by a repair polymerase


For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?

RNA Primer


For DNA polymerase to link nucleotides together as new strands of DNA the first nucleotide must be attached to what because DNA polymerase can only add nucleotides to a pre exsisting strand?

RNA Primer


What is the function of primer in PCR?

it synthesizes a single RNA primer at the 5' end of the leading end.