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Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
How a spectrophotometer measure change of colour?
A spectrophotometer measures the change in color by analyzing the amount of light absorbed or transmitted by a sample at different wavelengths. It quantifies the intensity of light absorbed by the sample and then converts this data into a measurable color change. This is done by comparing the absorbance spectrum of the sample to that of a reference.
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
What experimental variable is measured for change?
The experimental variable that is measured for change is typically called the dependent variable. It is the variable that is observed, measured, or tested in an experiment to see how it responds to changes in the independent variable.
How does dcpip in the hill reaction affect absorbance?
DCPIP is a blue colour when its oxidized and when it is reduced it turns into a colourless solution. DCPIP replaces NADPH the final electron acceptor in the light dependent reaction. So basically, as the reaction continues, the more DCPIP is reduced and the absorbance decreases.
What is the different between a control group and an experimental group in an experiment?
The control group does not change, while the experimental group is the variable you are changing.
Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
Why might the actual energy change for a reaction be different from the predicted energy change?
The actual energy change for a reaction might be different from the predicted energy change due to experimental errors, incomplete reactant conversion, side reactions, impurities in reactants, or inaccurate assumptions about reaction conditions. Kinetic factors such as reaction rates and temperature variations during the reaction can also contribute to discrepancies between predicted and actual energy changes.
How many variables are changed in a experiment?
Controlling for a variable is the act of deliberately varying the experimental conditions in order to take a single variable into account in the prediction of the outcome variable. Controlling tends to reduce the experimental error. A control is something that does not change in the experiment.
How a spectrophotometer measure change of colour?
A spectrophotometer measures the change in color by analyzing the amount of light absorbed or transmitted by a sample at different wavelengths. It quantifies the intensity of light absorbed by the sample and then converts this data into a measurable color change. This is done by comparing the absorbance spectrum of the sample to that of a reference.
What is the similarity between laser ablation and optical damage?
Optical damage can occur via different pathways, but if I imagine that with time, properties of thin films change (as a result of ambient conditions or the effects of the photons impinging on the surface) and hence absorbance may occur at the lasing wavelength. In the context of laser ablation, if absorbance, bond breakage, and ejection of material occurs then ablation has occured. This is signified by a audible report. From my experience, optical damage is typically not laser ablation but the result of gradual degradation of the optic - perhaps initiated by photons.
Why are the results of an investigation sometimes different?
Because conditions my change or be changed.
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
What are experimental variables?
things that you change every trial
Why absorbance should be taken in 750 nm in lowrys method?
Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.
State the first law of photochemistry and what is decadic absorbance?
only the radiation that is absorbed by reacting system can be effective in producing chemical change
What experimental variable is measured for change?
The experimental variable that is measured for change is typically called the dependent variable. It is the variable that is observed, measured, or tested in an experiment to see how it responds to changes in the independent variable.
