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To do this you also need to know the substance you are working with.

Lets take water as an example:

Lets say we have 100 milliliter of water at 20oC and you want to know how many moles this is.

Water has the molecular structure H2O.

So the molar mass is 1.007947*2 + 15.99943 = 18.015324 g/mol.

The density of water at 20oC is 0.998 g/cm3.

We can convert the volume to grams with the formula:

m = V * P

mass (g) = volume (cm3) * density (g/cm3)

So 100 milliliters of water * 0.998 g/cm3 = 99.8 grams of water.

We can turn this into moles by using the formula:

mm = m / n

molar mass = mass / moles

Which we can rewrite to:

n = m / mm

We know the mass (99.8 g) and the molar mass (18 g/mol).

Filling it in gives: 99.8 / 18 = 5.54 moles

So 100 milliliters of water at 20oC equals 5.54 moles.

This can be applied to most substances.

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