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purple
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What is the chemical indicator used to test protein?
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
Why does biuret reagent turn the skin brownish purple?
Biuret reagent react with the skin proteins.
What is the result when albumin solution pepsin and HCl are mixed with biuret reagent?
When albumin solution, pepsin, and HCl are mixed with biuret reagent, the resulting solution would likely turn purple due to the presence of proteins in the albumin solution. The biuret reagent reacts with peptide bonds in proteins, forming a purple complex. Pepsin is a protease enzyme that can break down proteins, potentially affecting the intensity of the purple color.
Does biuret's reagent turn lavender when heated?
No, biuret's reagent typically turns violet or pink when heated, not lavender. This color change is a characteristic reaction with proteins in which the copper ions in the reagent complex with peptide bonds, forming a colored complex.
What is formed when cuso4 n naoh are used for protein test?
When CuSO4 and NaOH are used for the protein test (Biuret test), the formation of a purple-colored complex indicates the presence of proteins. This color change occurs due to the interaction of the copper ions in CuSO4 with the peptide bonds in proteins, leading to the formation of a coordination complex that absorbs light in the visible range.
What is the chemical indicator used to test protein?
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
What color will a peptide bond be after using the Biuret test?
in biuret test the presence of peptide bonds gives a violet color. but it should be noted that a minimum of two peptide bonds should be present in the protein to get a positive answer to this test.
If biuret reagent turns purple what substance is present?
Proteins are present when biuret reagent turns purple. Biuret reagent reacts with peptide bonds in proteins, causing the color change.
What color does biuret reagent turn if lipid is present?
If lipids are present, the solution will turn a purple/violet color.
Why does biuret reagent turn the skin brownish purple?
Biuret reagent react with the skin proteins.
What is the result when albumin solution pepsin and HCl are mixed with biuret reagent?
When albumin solution, pepsin, and HCl are mixed with biuret reagent, the resulting solution would likely turn purple due to the presence of proteins in the albumin solution. The biuret reagent reacts with peptide bonds in proteins, forming a purple complex. Pepsin is a protease enzyme that can break down proteins, potentially affecting the intensity of the purple color.
What indicators are used for each molecule simple sugars starches lipids proteins?
Simple sugars: Benedict's solution test for reducing sugars. Starches: Iodine test, which turns blue-black in the presence of starch. Lipids: Sudan IV test, where lipids turn a red color. Proteins: Biuret test, leading to a color change from blue to purple in the presence of proteins.
Does biuret's reagent turn lavender when heated?
No, biuret's reagent typically turns violet or pink when heated, not lavender. This color change is a characteristic reaction with proteins in which the copper ions in the reagent complex with peptide bonds, forming a colored complex.
What color does distilled water turn with Benedict's reagent?
The water turns a pale blue. In other words stating that it doesnt contain protein
What is formed when cuso4 n naoh are used for protein test?
When CuSO4 and NaOH are used for the protein test (Biuret test), the formation of a purple-colored complex indicates the presence of proteins. This color change occurs due to the interaction of the copper ions in CuSO4 with the peptide bonds in proteins, leading to the formation of a coordination complex that absorbs light in the visible range.
Why diluted copper sulphate is used in biuret test?
Diluted copper sulfate is used in the Biuret test because it reacts with peptide bonds in proteins to form a colored complex. When proteins are present, the copper ions in the diluted solution turn from blue to violet, indicating the presence of peptide bonds. This test is particularly useful for detecting proteins in various samples due to its simplicity and sensitivity. The intensity of the color change correlates with the concentration of protein present.
An unknown sample is tested with both biuret reagent and benedicts reagent both test result in a blue color what has been learned and why are these called negative results?
That the unknown sample is not a monosaccharide and is does not contain peptide bonds (is not a protein). This is because they both produced negative results because Biuret tests positive in solutions that contain peptide bonds and will turn a violet color. Benedict reagent reacts to monosaccharides and will turn green-reddish orange when a monosaccharide is present. Neither of these things happened so the results are negative.
