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What else can I help you with?
What is the counter or secondary stain used in the Gram stain procedure?
The counter or secondary stain used in the Gram stain procedure is safranin.
What is the counter or secondary stain used in the acid-fast stain technique?
The counter or secondary stain used in the acid-fast stain technique is methylene blue.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What role does the secondary stain play in the process of gram staining?
The secondary stain in the process of gram staining helps to colorize bacteria that were not initially stained by the primary stain. This allows for differentiation between gram-positive and gram-negative bacteria based on their cell wall composition.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
Is counterstain a negative stain?
No, counterstain is not a negative stain. A counterstain is a secondary stain used in microscopy to color structures that were not stained by the primary stain, usually to provide contrast. Negative staining involves staining the background instead of the cells or structures of interest.
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What is secondary and counter stain?
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
How could you describe a stain instead of saying a big stain what could you say?
a huge stain. a jumbo stain. a large stain.
Is blood or egg an organic stain or protein stain?
its a protein stain
Why is a stain called a stain?
It's simple, because it's a stain.
