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Q: What step in glycolysis consists of a phosphorylation reaction in which ATP is the phosphate source?
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What reaction does the enzyme hexokinase catalyze?

First step of glycolysis- the phosphorylation of glucose to glucose-6-phosphate.


Substrate-level phosphorylation occurs within a metabolic pathway where sufficient energy is released by a given chemical reaction to drive the synthesis of ATP from ADP and phosphate Substrate-level?

both glycolysis and the Krebs cycle


Which is the committed step of glycolysis?

The committed step of glycolysis is the reaction catalyzed by phophofructokine (PFK) converting fructose-6-phosphate into fructose-1,6- bisphosphate. The reaction is irreversible and secondly, it's the only reaction peculiar to the glycolysis.


What is a coupled reaction using as an example the conversion of glucose to glucose-6-phosphate?

A coupled reaction is two reactions that occur together. One reaction is necessary for the other to occur.The conversion of glucose to glucose-6-phosphate is a good example. The first step that the cell takes in glycolysis (the beginning of the cellular respiration of glucose) is to convert glucose into glucose-6-phosphate. This phosphorylation requires an energy input, and therefore will not occur spontaneously.In the cell, both the necessary energy and the phosphate group are provided by a molecule of ATP. The free energy released by the conversion of ATP into ADP and a phosphate ion (Pi) is far greater than the energy required for the phosphorylation of glucose, and so, when the two reactions are coupled together, the phosphorylation of glucose goes ahead.To couple these reactions a hexokinase is required. This enzyme needs magnesium as a cofactor.


Name one reaction were ATP is required?

The first reaction of glycolysis, where glucose is phosphorylated (a phosphate group is added) to give glucose - 6 - phosphate requires ATP. This reaction is catalyzed by the enzyme hexokinase


2nd reaction of glycolysis. reactants, products, enzymes, reversibility?

Glucose-6-Phosphate to Fructose-6-Phosphate. reversible. Phosphogluctose isomerase.


Why might adding inorganic phosphate to a reaction mixture where glycolysis is rapidly proceeding help sustain the metabolic pathway?

The metabolic intermediates of glycolysis are phosphorylated.


What is subtrate level phosphorylation?

Oxidation of G3P occurs by the removal of hydrogen atoms are picked up by NAD+, and NADH+H+ results. Later the NADH will pass on electrons to the electron transport chain. Oxidation of G3P and subsequent substrtes result in four high energy phosphate groups and these are used to synthesize four ATP. So basically substrate-level ATP synthesis is when an enzyme passes a high-energy phosphate to ADP and ATP results.


Arsenate acts as uncoupling agent for oxidative phosphorylation?

Yes. During glycolysis, arsenate which has a similar structure to phosphate replaces it from glyceraldehyde-3-phosphate to form an unstable arsenate anhydride( 1-arsenato-3-phospho-d-glycerate) that hydrolyzes to form arsenate and 3-phosphoglycerate. In the absence of phosphate, ATP is not formed in the reaction. ADP-arsenate is formed from ADP and arsenate in the presence of succinate. The formation of easily hydrolyzable ADP-arsenate complex uncouples oxidative phosphorylation and causes arsenolysis.


4th reaction of glycolysis. reactants, products, enzymes, reversibility?

Fructose 1, 6-bisphosphate to dihydroxyacetone phosphate (DHAP) and Glyceraldehyde-3-Phosphate. Aldolase


What happens when ADP gains a phosphate to form ATP?

ATP or adenosine triphosphate. When ADP, adenosine diphosphate, gets a third phosphate group, it becomes ATP. ATP is the energy source of many reactions in the cell. When a reaction needs energy to occur, the enzyme that catalyzes the reaction also cleaves a molecule of ATP into ADP and phosphate. The energy of the phosphate bond is used to fuel the endothermic reaction. The ATP is regenerated (phosphorylation of ADP) in the glycolysis or another process that generates energy.


How many ATP molecule produced in Glycolysis?

There are two net molecules of ATP produced by substrate-level phosphorylation during glycolysis. (when one molecule of glucose is respired). Two are used to convert the glucose molecule to fructose, but four are released when pyruvate is made. However, the other products of glycolysis enable the Link Reaction, Krebs cycle and Oxidation Phosphorylation to happen, and these release a lot of ATP.