DNA polymerases
DNA Polymerase :)
Assuming this is regarding DNA replication or transcription, the enzyme helicase separates the two strands.
RNA polymerase is the enzyme that connect the new nucleotides together and proofreads them.
Enzyme-substrate specificity means that a substrate can fit into an enzyme similar to a key fitting into a lock. The active site of the enzyme is what determines its specificity. An enzyme can hence catalyze a reaction with a specific substrate, such as amylase catalyzing starch molecules. During these reactions, the substrate is held in a precise optimum position to create and break bonds, catalyzing the molecule.
It is in the mitochodria and speeds up the formation of ATP by breaking down ATP into ADP + energy. Muscle cells have many more mitochrondia than other cells.
an active site in an enzyme is the area that breaks the bond in its substrate. E.g. a maltose molecule's glycocide bond is broken by the active site in a maltase enzyme.
pyruvate
creatine kinase
Intracellular enzymes act inside cells and are responsible for catalyzing reactions that are occurring in metabolic pathways in the chloroplast.
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
The inhibitor.
Protease for catalyzing proteins.
At high temperatures, DNA denatures into single strands. A temperature around 95 degrees should work.
Helicases and gyrases both function to uncoil the supercoiling of the two DNA strands. Moreover, gyrase is responsible for adjusting the tension in the two strands so that they don't snap while unraveling.
ligase
DNA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers with nucleotides, and DNA Ligase which joins fragments of DNA together.
DNA Polymerase
Assuming this is regarding DNA replication or transcription, the enzyme helicase separates the two strands.