take for an example there are many beetles on a mountain there will also be many sub groupssince the beetles wont travel far away for food or mate and hence will mate in a small region forming many sub groups.
now a beetl somehow (carried by crow)reaches anothe sub group .it will surely mate with a female there and there will bw a gene flow from one sub population to another .
now for ex a river is formed between 2 sub populations then this type of gene flow will bwcome even less .over years the 2 sub population will mate and both genetic drift and natural selection will create big changes in the 2 populations ,
the changes will become so big tha t the 2 polations will not be able mate with each other .creating two diff seicies
this is called genetic isolation or speciation
DNA isolation is the process of deriving a DNA from a sample with the help of various chemical reactions. The first DNA isolation was done in 1869 by Friedrich Miescher.
Digests RNA molecules
Protective (regular) isolation- keeps germs inside clients room Regular(routine) isolation- keeps germs outside client's room
That would be geographic isolation and reproductive isolation. Both could lead to speciation.
Traits are passed by DNA.
DNA polymerases
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
it act as as a cationic detergent for the isolation dna from the given sample
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
The DNA fragments comes from the method of DNA isolation.
to precipitate extracted DNA
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
It solubilizes lipids and a lot of proteins to remove them from the DNA.
heparin may be extrected along with DNA
70% ethnol is used for the dna isolation becuse it makes hydrogen bonding with the water molecules and makes DNA hydrophopic so that it pricipitated.
check the absorption of the extracted material at 260nm... After DNA isolation, there is the possibility of protein contamination. If there are small changes in the way the isolation is done and the amount of detergents added and the centrifugation speeds, they could affect the final purity of the isolated DNA. Another common contaminant is RNA. Once the DNA has been purified, a small amount of the sample is taken for spectrophotometric analysis. Here, the sample is exposed to light of 260 and 280nm wavelength and the absorbency is noted. The ratio of the absorbency at these two wavelengths is calculated. If the ratio is between 1.8 and 2.0, then the DNA is considered pure for further applications. If not, then the isolation protocol has to be changed or the reagents have to be replaced in toder to obtain pure DNA