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take for an example there are many beetles on a mountain there will also be many sub groupssince the beetles wont travel far away for food or mate and hence will mate in a small region forming many sub groups.
now a beetl somehow (carried by crow)reaches anothe sub group .it will surely mate with a female there and there will bw a gene flow from one sub population to another .
now for ex a river is formed between 2 sub populations then this type of gene flow will bwcome even less .over years the 2 sub population will mate and both genetic drift and natural selection will create big changes in the 2 populations ,
the changes will become so big tha t the 2 polations will not be able mate with each other .creating two diff seicies
this is called genetic isolation or speciation
What else can I help you with?
What does RNase solution do in the Isolation of genomic DNA from Negative bacteria?
Digests RNA molecules
What is the Differentiate between protective isolation and regular isolation?
Protective (regular) isolation- keeps germs inside clients room Regular(routine) isolation- keeps germs outside client's room
What are the two main types of isolation?
That would be geographic isolation and reproductive isolation. Both could lead to speciation.
How are traits passed from parents to their offspring?
Traits are passed by DNA.
What enzyme is responsible for catalyzing the formation of the two new DNA strands?
DNA polymerases
What is the role of carrier RNA in DNA isolation?
Carrier RNA is used in DNA isolation to help precipitate and recover DNA more efficiently. It acts as a carrier for the DNA during precipitation, helping to aggregate the DNA molecules together for ease of isolation. This improves DNA recovery and purity during the isolation process.
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
Role of sucrose in DNA isolation from human blood?
Sucrose is used in DNA isolation from human blood as a protective agent to help maintain the integrity of the DNA during the isolation process. It helps to stabilize the DNA by providing a protective barrier against enzymes and other degradation factors present in the blood sample. Additionally, sucrose can aid in the separation of DNA from other cellular components during the isolation procedure.
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
Potassium chloride is used in Tkm1 buffer to help maintain the appropriate ionic strength for DNA isolation. It helps to stabilize the DNA through proper salt concentration, assisting in the precipitation of DNA during the isolation process.
What is the need for centrifuge in DNA isolation?
Centrifuge is needed in DNA isolation to separate the DNA from other cellular components such as proteins, RNA, and cell debris based on their size and density differences. By spinning the sample at high speeds, the centrifuge helps to pellet the DNA at the bottom of the tube, allowing for the isolation and extraction of pure DNA.
Why heparin can not be used in DNA isolation methods?
heparin may be extrected along with DNA
How will we know after DNA isolation if the material is DNA or not or how will we identified the DNA in DNA isolation proceess?
check the absorption of the extracted material at 260nm... After DNA isolation, there is the possibility of protein contamination. If there are small changes in the way the isolation is done and the amount of detergents added and the centrifugation speeds, they could affect the final purity of the isolated DNA. Another common contaminant is RNA. Once the DNA has been purified, a small amount of the sample is taken for spectrophotometric analysis. Here, the sample is exposed to light of 260 and 280nm wavelength and the absorbency is noted. The ratio of the absorbency at these two wavelengths is calculated. If the ratio is between 1.8 and 2.0, then the DNA is considered pure for further applications. If not, then the isolation protocol has to be changed or the reagents have to be replaced in toder to obtain pure DNA
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What does DNA isolation used for?
Gene sequencing and gene cloning
Why use chloroform in DNA isolation?
DNA is soluble in chloroform more than water. So we use it.
Role of phenol chloroform in DNA isolation?
Phenol chloroform is used in DNA isolation to separate DNA from other cellular components. It helps to denature proteins and lipids, allowing DNA to partition into the aqueous phase while other cellular debris remains in the organic phase. This method helps to purify DNA for downstream applications like PCR or sequencing.
What are the function of sodium acetate in dna isolation?
Sodium acetate is used in DNA isolation as a salt to promote DNA precipitation, helping to remove contaminants and impurities from the DNA sample. It is commonly used in combination with ethanol to precipitate DNA from solution, allowing for the extraction and purification of DNA for further analysis. Sodium acetate also helps to maintain the appropriate pH level for DNA precipitation to occur effectively.
