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If you want to calculate the concentration of haemoglobin, you need to convert haemoglobin into cyanmethaemoglobin, because cyanmethaemoglobin can be measured colourimetrically.
You need
- whole blood
- EDTA , and
- Drabkin's reagent.
As Haemoglobin reacts with potassium ferricyanide (in the Drabkin's reagent), haemoglobin will convert into methaemoglobin.
Methaemoglobin will react with potassium cyanide (also in the Drabkin's reagent), and you will get cyanmethaemoglobin.
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Hb + potassium ferricyanide → methaemoglobin
Methaemoglobin + potassium cyanide →cyanmethaemoglobin
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Cyanmethaemoglobin can be measured by using a spectrometer (absorbance at 540 nm.).
Using the equation, it will give you the concentration of Haemoglobin.
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Hb (g/dL) = Abs. of test sample / abs. of standard x concentration of standard.
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(The concentration is directly proportional to the absorbance)
What else can I help you with?
Why do you need science?
You need science to live you need science to learn and be creative.
What do you need to breathe in and out?
You need to breathe in oxygen and you need to breathe out carbon dioxide.
Why and what do you need water for?
the most important two things we need are water and sun, we need water to live
Why do plants and animals need different things?
Plants and animals need different things because they have different needs. Plants need sunlight because they make their own food and animals need plants.
What equipment do you need to go with a Bunsen burner?
You need safety goggles
What is the color of methemoglobin?
Methemoglobin is brownish in color.
Hemoglobin that has undergone oxidation of the iron is known as?
methemoglobin
Why was your blood dark brown after your blood draw?
Could be Methemoglobin. This is very rare.
What is methanoglobnemia?
Methanoglobnemia is mispelled, it's actually methemoglobinemia Methemoglobinemia is a blood disorder in which an abnormal amount of methemoglobin -- a form of hemoglobin -- is produced. Hemoglobin is the molecule in red blood cells that distributes oxygen to the body. Methemoglobin cannot release oxygen. In methemoglobinemia, the hemoglobin is unable to release oxygen effectively to body tissues.
What has the author George H Barrows written?
George H. Barrows has written: 'Methemoglobin reduction in vitamin K deficient chicks'
What precaution or side effects should you be aware of when administering cyanide antidotes?
Sodium nitrite can increase methemoglobin levels, which will decrease blood oxygenation
Do people have blue skin?
This question may be in reference to the Tauregs of Morocco who are known as the Blue People because of the blue clothing they wear or something known methemoglobin.
Do some people have blue skin?
This question may be in reference to the Tauregs of Morocco who are known as the Blue People because of the blue clothing they wear or something known methemoglobin.
What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
What are the causes of the conginital form of mehtemoglobinemia?
One cause is a defect in the body's systems to reduce methemoglobin to hemoglobin. The other cause is a mutant form of hemoglobin called hemoglobin M that cannot bind to oxygen.
When was Methemoglobinemia discovered?
Methemoglobinemia was first described in 1943 by a physicians Alfred Stengel and Albert Mason of the University of Pennsylvania. They identified it as a condition where hemoglobin is unable to carry oxygen effectively due to the presence of excess methemoglobin in the blood.
What is Haemin Crystals?
Haemin crystals are needle-like structures that form when hemoglobin is oxidized and turns into methemoglobin. These crystals are commonly seen in blood smears from patients with certain types of hemolytic anemias or hemoglobinopathies. They are visualized under a microscope and their presence can aid in diagnosing these conditions.
