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If you want to calculate the concentration of haemoglobin, you need to convert haemoglobin into cyanmethaemoglobin, because cyanmethaemoglobin can be measured colourimetrically.
You need
- whole blood
- EDTA , and
- Drabkin's reagent.
As Haemoglobin reacts with potassium ferricyanide (in the Drabkin's reagent), haemoglobin will convert into methaemoglobin.
Methaemoglobin will react with potassium cyanide (also in the Drabkin's reagent), and you will get cyanmethaemoglobin.
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Hb + potassium ferricyanide → methaemoglobin
Methaemoglobin + potassium cyanide →cyanmethaemoglobin
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Cyanmethaemoglobin can be measured by using a spectrometer (absorbance at 540 nm.).
Using the equation, it will give you the concentration of Haemoglobin.
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Hb (g/dL) = Abs. of test sample / abs. of standard x concentration of standard.
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(The concentration is directly proportional to the absorbance)
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Why do you need science?
You need science to live you need science to learn and be creative.
What do you need to breathe in and out?
You need to breathe in oxygen and you need to breathe out carbon dioxide.
Why and what do you need water for?
the most important two things we need are water and sun, we need water to live
Why do plants and animals need different things?
Plants and animals need different things because they have different needs. Plants need sunlight because they make their own food and animals need plants.
What equipment do you need to go with a Bunsen burner?
You need safety goggles
Hemoglobin that has undergone oxidation of the iron is known as?
methemoglobin
Why was your blood dark brown after your blood draw?
Could be Methemoglobin. This is very rare.
What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
What is methanoglobnemia?
Methanoglobnemia is mispelled, it's actually methemoglobinemia Methemoglobinemia is a blood disorder in which an abnormal amount of methemoglobin -- a form of hemoglobin -- is produced. Hemoglobin is the molecule in red blood cells that distributes oxygen to the body. Methemoglobin cannot release oxygen. In methemoglobinemia, the hemoglobin is unable to release oxygen effectively to body tissues.
What has the author George H Barrows written?
George H. Barrows has written: 'Methemoglobin reduction in vitamin K deficient chicks'
The bonding of CO2 to hemoglobin produces?
The bonding of carbon dioxide to hemoglobin produces methemoglobin. This is an important chemical in the immune system of the human body.
What precaution or side effects should you be aware of when administering cyanide antidotes?
Sodium nitrite can increase methemoglobin levels, which will decrease blood oxygenation
Do people have blue skin?
This question may be in reference to the Tauregs of Morocco who are known as the Blue People because of the blue clothing they wear or something known methemoglobin.
Do some people have blue skin?
This question may be in reference to the Tauregs of Morocco who are known as the Blue People because of the blue clothing they wear or something known methemoglobin.
What are the causes of the conginital form of mehtemoglobinemia?
One cause is a defect in the body's systems to reduce methemoglobin to hemoglobin. The other cause is a mutant form of hemoglobin called hemoglobin M that cannot bind to oxygen.
What determine whether blood is bright red or dull brick red?
The color comes from hemoglobin and the hemoglobin changes color depending upon what is bound to it. Oxyhemoglobin (hemoglobin with oxygen bound to it), deoxyhemoglobin (hemoglobin with no oxygen bound to it) is somewhat bluish. Arterial blood, which has been to the lungs and is on its way to the tissues, is usually fairly saturated with oxygen (anywhere from 95-100% of the hemoglobin is bound to oxygen), and so it looks bright red. Blood in the veins, coming back from the tissues, is less saturated (perhaps 60-80%), and so it appears darker. If there is enough unbound hemoglobin in the blood, it may actually take on a bluish hue, and that is why someone may appear blue if they are not getting oxygen. Carbon monoxide also binds to hemoglobin and results in an even brighter red color than when oxygen binds. Someone with carbon monoxide poisoning may have even brighter red blood and may have a bright red coloration to the lips and fingernails. It is also possible for the iron in hemoglobin to be changed from the ferrous (2+) to ferric (3+) state and the result is methemoglobin, which is a more brownish compound. There is normally a very small amount of methemoglobin circulating in the blood and this can be increased by exposure to certain chemicals (in particular, certain nitrogen compounds). Interestingly, methemoglobin is capable of binding cyanide, so the "antidote" for cyanide poisoning is a nitrogen compound that converts hemoglobin to methemoglobin, which then binds up free cyanide in the blood. Under normal circumstances, the blood contains primarily oxyhemoglobin and deoxyhemoglobin.
Why do alpha streptococci produce green color on blood agar?
All of the different streptococci are organized into three groups based on how they break down red blood cells, a process called hemolysis. The group of streptococci that perform alpha hemolysis are called alpha streptococci. In alpha hemolysis, the hydrogen peroxide produced by the streptococci oxidizes the blood's hemoglobin, turning it into methemoglobin, which is green in color.
