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An advantage of using reverse transcriptase to prepare a gene for cloning is that it allows for the synthesis of complementary DNA (cDNA) from messenger RNA (mRNA). This process enables researchers to obtain a gene that is expressed in a specific tissue or under certain conditions, effectively capturing only the coding sequences without introns. Additionally, cDNA can be easily amplified and cloned, facilitating further studies on gene function and expression.
What else can I help you with?
In advantage of using reverse transcriptase to prepare a gene for cloning is that?
the resulting DNA strand will lack introns
How did scientists convent information when using reverse transcriptase in insulin research?
Scientists used reverse transcriptase in insulin research by first isolating messenger RNA (mRNA) from cells that produce insulin. The reverse transcriptase enzyme then converted this mRNA into complementary DNA (cDNA), which could be used for further analysis and cloning. This allowed researchers to study the genetic basis of insulin production and potentially manipulate it for various applications.
Can reverse transcriptase be denatured?
Yes, reverse transcriptase can be denatured under certain conditions. High temperature or extreme pH levels can disrupt the structure of reverse transcriptase, rendering it inactive. Denaturation of reverse transcriptase can prevent it from catalyzing the conversion of RNA into DNA during the process of reverse transcription.
What enzyme do viruses use to produce m-RNA?
reverse transcriptase
What enzyme is used in reverse transcription?
Reverse transcriptase is the enzyme used in reverse transcription to generate complementary DNA (cDNA) from an RNA template.
What does a eukaryotic cell use to make reverse transcriptase?
A eukaryotic cell uses its own enzymes to make reverse transcriptase.
What is formed when reverse transcriptase is used on a strand of mRNA?
A strand of DNA
What enzyme is contained in retroviruses?
Retroviruses contain an enzyme called reverse transcriptase, which helps transcribe the viral RNA genome into DNA once inside the host cell. This DNA then integrates into the host cell's genome, allowing the virus to replicate and persist within the host.
Which Enzyme is capable of reverse transcription?
Rna reverse transcriptase.
What does transcriptase use to form DNA?
Reverse transcriptase use mRNA to form DNA. mRNA
What enzyme is unique to retrovirus?
Reverse transcriptase.
Why are using reverse transcriptase necessary for finding a way to make artificial insulin?
Reverse transcriptase is necessary for making artificial insulin because it converts RNA into complementary DNA (cDNA). Since the insulin gene is often expressed in the form of mRNA in cells, reverse transcriptase enables researchers to create a stable, DNA version of this gene. This cDNA can then be inserted into plasmids for cloning and expression in bacteria or yeast, allowing for the production of recombinant insulin. This process is crucial for generating large quantities of insulin for therapeutic use.
