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Agar must be sterilized before using because if it isn't. it could grow almost anything from the environment. It would spoil your ability to say which microbe you have being given. I think it would be like saying that you would be breaking the chain of evidence. I know that you would not be able to say for sure what you grew "out".
What else can I help you with?
Why do you heat agar in flask before dispensing into tubes to make agar slants?
If you are talking about heating the agar before using it, then you are not talking about making the agar from scratch. You are talking about agar that has already been prepared and has been allowed to solidify in a flask. Heating is necessary because the agar must go from its solid state to a liquid state so that it can bePOURED into a tube where it can solidify inside of the slanted position of the tube.
Why must warm agar be cooled down before the tubes are inoculated?
Warm agar must be cooled down before inoculation to prevent damaging the microorganisms being introduced. If the agar is too hot, it can kill or inhibit the growth of the microbes, leading to inaccurate results. Additionally, cooling the agar allows it to solidify properly, providing a suitable medium for the organisms to grow and form colonies. This ensures optimal conditions for microbial growth and experimentation.
Why the plant must be cut into small pieces before culturing on potato dextrose agar?
A plant must be cut into small pieces before culturing, such as potato dextrose agar, because it is easier to handle in a cheese cloth. You must let the potato boil in distilled water.
Why must the tubes be inoculated before agar is poured?
Inoculating the tubes with the desired microorganism before pouring agar ensures even distribution of the microorganism in the agar, promoting growth and forming distinct colonies. This step is crucial for obtaining pure cultures and preventing contamination.
Why must the temperature of the agar be no lower than 45C and no higher than 50C when being poured into the plates?
the bacteria will dentaure and die off
Plastic instruments must be sterilized by?
soaking them for 10 hours in Glutaraldehyde.
Why must a powdered complex medium be sterilized immediately after being stored for months?
Because the microbes will start to digest it almost immediately, and their products will change the medium properties.
Why cooling the inoculating instrument prior to obtaining the inoculum?
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
What specific medium must be use in testing the effectiveness of antibiotics?
A growth medium must be used. The most common is Mueller-Hinton agar, but potato dextrose agar or other growth media could also be used.
How do you prepare fermentation?
You must make a saline solution and fully submerse the item you are fermenting. Everything must be sterilized beforehand to prevent bad bacteria from growing.
Why must a straight inoculating needle be used when inoculating an agar deep tube?
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
How many years of service must an officer or NCO have before being selected for casualty assistance officer?
An officer or NCO must have 6years of service before being selected as a casualty assistance officer. They must also be mature and trustworthy.
