bluish color if pentose is present.
Bial's orcinol test is used to detect the presence of pentoses (such as ribose and deoxyribose) in samples. When pentoses react with orcinol in the test, a blue-green color is produced. This test is commonly utilized in the analysis of carbohydrates, nucleic acids, and other biological molecules.
300 mg of orcinol in 100 ml of conc. HCl and 0.25 ml of ferric chloride solution (10g / 100ml ).
Hydrolyzed DNA in Bial's test produces a blue-green color due to the reaction of deoxyribose sugars with orcinol reagent and sulfuric acid. This color change indicates the presence of pentoses in the sample.
The specific test for pentoses is the Bial's test. This test involves heating the sugar with orcinol and an acidic solution, resulting in a blue-green color if pentoses are present.
discussion abut bial test ??
Bial's Test is primarily used to detect the presence of pentoses, which are found in RNA but not in DNA. When subjected to Bial's Test, RNA will produce a positive result due to its ribose sugar, while DNA, which contains deoxyribose, will not react in the same way. Therefore, while Bial's Test can indicate the presence of RNA over DNA, it does not provide a direct structural distinction between the two nucleic acids.
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
The aniline acetate test for xylose is more reliable than Bial's test because it specifically detects the aldehyde group in xylose, providing a more accurate and sensitive result. Bial's test, on the other hand, is less specific as it can give false positive results with other sugars containing ketone groups.
Glucuronic acid would produce a violet color during the Bial's test. The violet color indicates the presence of pentoses or uronic acids in the solution.
The population of Bial is 2,009.
Pedro Bial is 192 cm.