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In the osazone test, reducing sugars like glucose or fructose react with phenylhydrazine to form crystalline derivatives called osazones. While starch itself is a polysaccharide and does not directly participate in this reaction, when starch is hydrolyzed into its constituent glucose units, these reducing sugars can then react with phenylhydrazine to form osazones. Sucrose, being a non-reducing sugar, does not form osazones unless it is first hydrolyzed into glucose and fructose. Thus, it is the monosaccharides released from starch and sucrose that contribute to the formation of the crystalline osazones.

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1mo ago

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