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Explain the function of replication?
DNA replication requires the opening of the 'zipped up' DNA strand. This is so a 'new' strand of DNA can be inserted and have a template strand to 'read' off. DNA polymerase analyses the bases on the template strand and adds each complementary base to synthesise the 'new' strand. In order for DNA polymerase to be able to do this the DNA has to be opened up by helicase to reveal the bases of the template strand. The unzipping of the DNA by helicase forms the replication fork. Thus the function of the replication fork is to reveal template strands for DNA replication to actually occur.
What are complimentary way DNA's nitrogen bases bond together allow the molecule to make an identical copy If a mistake is made in the replication of DNA it is called a?
DNA's nitrogen bases bond together through hydrogen bonding, with adenine pairing with thymine and cytosine pairing with guanine. This complementary base pairing allows DNA to make an identical copy during replication. A mistake in the replication process is called a mutation.
Does DNA replication start at only one place in the DNA?
What do you mean by one area? If by one area you mean does it start at one area then stop and continue on another area then no, DNA replication is continuous and when it takes place it is the most important thing going on in the cell. The cells resources are directed at this process * DNA replication means copying the entire DNA molecule, so it involves the entire molecule, but not all at the same time. In bacteria (prokaryotic cells) replication begins at one point in the molecule and continues all the way round the circular molecule.Beginning at just one place would take too long in eukaryotic cells; someone has calculated that our longest chromosomes would take about a fortnight to replicate, and we cannot wait that long! So replication begins at a number of sites along the length of the DNA molecule. At each replication site, as the DNA strands are separated there is a bulge in the molecule called a replication bubble. As replication proceeds these bubbles become longer, and eventually they merge into one another and the job is done.
What is the difference between the Helicase and DNA polymerase enzme?
The helicase in a way unzipps the wound DNA. DNA Polymerase then matches the nucleotide bases with free floating one so that A matches with T (or in RNA U) and G matches with C. Creating two new strands of DNA and completing DNA replication.
What is the difference between replication of DNA and amplifcation of DNA in PCR?
DNA cloning is where you take a piece of DNA and put it in a host cell so that every time the host cell replicates, its daughter cells will have that exact copy of DNA. DNA amplification is just taking a piece of DNA and making copies of it, like in the process of PCR. it is not inside a host cell. another way to think of it: you can amplify a gene--make a bunch of copies of it, and then clone it (by putting it in a cell and once that cell replicates each daughter cell has a copy of that DNA). you don't need to amplify anymore in cloning, you already did that before.
In what way are DNA replication and PCR related?
DNA replication and PCR are related in that they both involve the process of copying DNA. DNA replication occurs naturally in cells to create new copies of DNA for cell division, while PCR is a laboratory technique that amplifies specific DNA sequences by replicating them in a controlled environment.
What is the process by which a DNA molecule is copied called?
The process by which a DNA molecule is copied is called DNA replication. During DNA replication, the two strands of the double helix separate and each strand serves as a template for the synthesis of a new complementary strand. This results in two identical copies of the DNA molecule.
How do DNA bases pair up in the process of DNA replication?
During DNA replication, the DNA bases pair up in a specific way: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). This pairing is essential for accurately copying the genetic information stored in DNA.
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
Why does a new DNA strand elongate only in the 5' to 3' direction during replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
Why are there many replication forks during DNA replication?
The DNA polymerase enzymes can only operate from the 3' end of the chain to the 5' end, not the other way. The two helices that make up the double helix of DNA are oriented oppositely, as shown in the diagram above, making the DNA polymerase enzymes move in opposite directions.
Explain the function of replication?
DNA replication requires the opening of the 'zipped up' DNA strand. This is so a 'new' strand of DNA can be inserted and have a template strand to 'read' off. DNA polymerase analyses the bases on the template strand and adds each complementary base to synthesise the 'new' strand. In order for DNA polymerase to be able to do this the DNA has to be opened up by helicase to reveal the bases of the template strand. The unzipping of the DNA by helicase forms the replication fork. Thus the function of the replication fork is to reveal template strands for DNA replication to actually occur.
What part of the cell does replication occur?
Replication occurs in the nucleus of a eukaryotic cell and in the cytoplasm of a prokaryotic cell. In eukaryotic cells, DNA replication takes place in the nucleus where the genetic material is located, while in prokaryotic cells, replication occurs in the cytoplasm since they lack a true nucleus.
What are complimentary way DNA's nitrogen bases bond together allow the molecule to make an identical copy If a mistake is made in the replication of DNA it is called a?
DNA's nitrogen bases bond together through hydrogen bonding, with adenine pairing with thymine and cytosine pairing with guanine. This complementary base pairing allows DNA to make an identical copy during replication. A mistake in the replication process is called a mutation.
What enzymes check for mistakes in replication?
DNA polymerase is the main enzyme responsible for checking for mistakes during DNA replication. It has a proofreading function that helps to correct errors in base pairing. Additionally, mismatch repair enzymes are also involved in identifying and repairing any remaining errors after replication.
Why is DNA replication called semi conservative?
DNA replication of one helix of DNA results in two identical helices. If the original DNA helix is calledthe "parental" DNA, the two resulting helices can be called "daughter" helices. Each of these two daughter helices is a nearly exact copy of the parental helix (it is not 100% the same due to mutations).DNA creates "daughters" by using the parental strands of DNA as a template or guide. Each newly synthesized strand of DNA (daughter strand) is made by the addition of a nucleotide that is complementary to the parent strand of DNA. In this way, DNA replication is semi-conservative, meaning that one parent strand is always passed on to the daughter helix of DNA.
Does DNA replication start at only one place in the DNA?
What do you mean by one area? If by one area you mean does it start at one area then stop and continue on another area then no, DNA replication is continuous and when it takes place it is the most important thing going on in the cell. The cells resources are directed at this process * DNA replication means copying the entire DNA molecule, so it involves the entire molecule, but not all at the same time. In bacteria (prokaryotic cells) replication begins at one point in the molecule and continues all the way round the circular molecule.Beginning at just one place would take too long in eukaryotic cells; someone has calculated that our longest chromosomes would take about a fortnight to replicate, and we cannot wait that long! So replication begins at a number of sites along the length of the DNA molecule. At each replication site, as the DNA strands are separated there is a bulge in the molecule called a replication bubble. As replication proceeds these bubbles become longer, and eventually they merge into one another and the job is done.
