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To perform a pure culture, you will need culture media (agar plates or broth), inoculation loop or needle, incubator, and the sample containing the microorganism you want to culture. These materials will help create a controlled environment for the growth of a single species of microorganism.

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1y ago

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What is a pure culture?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one


Is determining the conditions required for materials to burn applied or pure chemistry?

I suppose that this is an example of applied chemistry.


What is a broth culture?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one


Can a Pure culture prepared from mixed broth or mixed agar slant?

To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate methodThe next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof


What is a broth?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one


Why obtain pure culture of bacteria from a mixed culture?

i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture


Is pure culture having same meaning of microbial culture?

Yes, a pure culture refers to a microorganism culture that contains only a single strain or species, free from contamination. Microbial culture is a broader term that can refer to any type of culture of microorganisms, including pure cultures or mixed cultures containing multiple strains or species.


What is the difference between pure culture and auxanic culture?

A pure culture contains only one type of microorganism, while an auxanic culture is a type of pure culture that has been enriched to promote growth of specific microorganisms, typically for research purposes. Auxanic cultures may contain growth factors or nutrients that allow the targeted microorganism to thrive.


Could you distinguish between axenic culture and pure culture?

An axenic culture refers to a culture containing only one species of microorganism without any other contaminants present. A pure culture, on the other hand, refers to a culture containing only one specific strain or species of microorganism, but it may still have contaminants present. In essence, axenic culture is free of any contaminants, while a pure culture may have contaminants other than the desired microorganism.


How would you define pure culture sterile and contamination in the isolation and purification of bacteria?

A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.


How would you define pure culture and sterile and contamination in the isolation and purification of bacteria?

A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.


What are the prevention pure culture contamination?

Some ways to prevent pure culture contamination include maintaining aseptic technique, using sterile equipment and reagents, working in a clean environment, practicing good personal hygiene, and storing cultures properly. Regularly cleaning work surfaces and equipment, properly disposing of biohazard materials, and promptly addressing any spills or accidents can also help prevent contamination.