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Why is a chromatogram like a chemical fingerprint?
A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.
What condition should use the standard addition method?
The standard addition method is typically used in analytical chemistry when analyzing samples with unknown concentrations, where a known amount of standard solution is added to the sample to create a series of solutions with different concentrations. This method is particularly useful when the matrix of the sample interferes with other quantitative methods.
What would happen to the pigment spots if the chromatogram was left running for a long time?
If the chromatogram is left running for an extended period, the pigment spots may continue to separate as the solvent carries them further along the stationary phase. This could lead to increased resolution between the different pigments, but if left too long, the spots may become too diffuse and potentially merge into one another. Additionally, excessive time might cause the solvent front to reach the end of the chromatogram, resulting in a loss of resolution and clarity.
Would a pure substance show more than one spot in the chromatogram?
No, a pure substance would typically show only one spot in a chromatogram. This is because a pure substance consists of only one type of molecule, leading to a single unique retention factor (Rf value) during the separation process. If more than one spot is observed, it indicates the presence of impurities or a mixture of substances.
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Would a pure substance show more than one color or spot in a chromatogram. explain?
No, a pure substance would only show a single color or spot in a chromatogram because it consists of only one compound without any impurities. Each compound present in a sample will appear as a distinct spot with a unique color in the chromatogram.
What would happen if the beaker is left uncovered during the development of the chromatogram?
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
How many peaks in hexanes gc chromatogram?
The number of peaks in a hexanes GC chromatogram can vary depending on the specific composition of the sample being analyzed. Hexanes is a non-polar solvent often used in gas chromatography, and if the sample contains various volatile organic compounds, multiple peaks will appear corresponding to each component. Typically, pure hexanes would yield a single peak, while a mixture could show several peaks representing different analytes. The exact number will depend on the complexity of the sample.
Why it is necessary to spray the chromotogram with ninhydrin?
Spraying the chromatogram with ninhydrin is necessary because ninhydrin reacts with amino acids and proteins, resulting in the formation of colored complexes. This reaction allows for the visualization of these compounds on the chromatogram, which would otherwise be invisible. By revealing the presence and quantity of amino acids, researchers can analyze the separation and identify specific components in the sample.
Why is a chromatogram like a chemical fingerprint?
A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.
What would adding water to a urine sample given to police for suspected drink driving?
what effect would adding water to a urine sample have on it for suspected drink driving
Sample research questions?
A research question should be concise and show cause and effect. An example would be. Does weather have an effect on increased real estate sales?
What topic is too broad for a cause-and-effect essay?
A topic like "climate change" may be too broad for a cause-and-effect essay because it encompasses a wide range of factors and consequences that would be challenging to cover comprehensively in a short essay. Breaking it down into more specific aspects, such as the impact of deforestation on climate change, would make for a more focused and manageable essay.
What condition should use the standard addition method?
The standard addition method is typically used in analytical chemistry when analyzing samples with unknown concentrations, where a known amount of standard solution is added to the sample to create a series of solutions with different concentrations. This method is particularly useful when the matrix of the sample interferes with other quantitative methods.
What would be the effect of using FTM tubes with broad pink zone?
Using FTM (Fluid Thioglycollate Medium) tubes with a broad pink zone indicates the presence of a significant amount of dissolved oxygen, suggesting that the medium is not effectively reducing oxygen levels. This can lead to the growth of aerobic bacteria, which may mask the growth of anaerobic bacteria that would typically thrive in a more reduced environment. Consequently, the results of microbial cultures may be skewed, potentially leading to false interpretations regarding the sample's microbial composition. Properly maintaining anaerobic conditions is crucial for accurate microbiological analysis.
What is the difference between reagent blank and sample blank?
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
What is correct your friend lives in Broad Street or your friend lives at Broad Street?
Your friend lives on Broad Street is the correct form.You would say: Your friend lives in Albany.You would say: Your friend lives at the corner of Broad Street and River Road.
