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A broad sample would result in peak broadening on the chromatogram. This can be caused by factors such as sample dispersion, slow diffusion rates, or poor column efficiency. Broad peaks can lead to decreased resolution and difficulty in accurately determining peak parameters.

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1y ago

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Why is a chromatogram like a chemical fingerprint?

A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.


What condition should use the standard addition method?

The standard addition method is typically used in analytical chemistry when analyzing samples with unknown concentrations, where a known amount of standard solution is added to the sample to create a series of solutions with different concentrations. This method is particularly useful when the matrix of the sample interferes with other quantitative methods.


What would happen to the pigment spots if the chromatogram was left running for a long time?

If the chromatogram is left running for an extended period, the pigment spots may continue to separate as the solvent carries them further along the stationary phase. This could lead to increased resolution between the different pigments, but if left too long, the spots may become too diffuse and potentially merge into one another. Additionally, excessive time might cause the solvent front to reach the end of the chromatogram, resulting in a loss of resolution and clarity.


What would happen if the Spot of origin is lowered into the solvent in chromatography?

If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!


How much of a 100 gram sample would remain unchanged after two hours?

This would depend on the specific sample and its stability. Without additional information, it is not possible to determine how much of the sample would remain unchanged after two hours.

Related Questions

Would a pure substance show more than one color or spot in a chromatogram. explain?

No, a pure substance would only show a single color or spot in a chromatogram because it consists of only one compound without any impurities. Each compound present in a sample will appear as a distinct spot with a unique color in the chromatogram.


What would happen if the beaker is left uncovered during the development of the chromatogram?

Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.


Why is a chromatogram like a chemical fingerprint?

A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.


What would adding water to a urine sample given to police for suspected drink driving?

what effect would adding water to a urine sample have on it for suspected drink driving


Sample research questions?

A research question should be concise and show cause and effect. An example would be. Does weather have an effect on increased real estate sales?


What topic is too broad for a cause-and-effect essay?

A topic like "climate change" may be too broad for a cause-and-effect essay because it encompasses a wide range of factors and consequences that would be challenging to cover comprehensively in a short essay. Breaking it down into more specific aspects, such as the impact of deforestation on climate change, would make for a more focused and manageable essay.


What condition should use the standard addition method?

The standard addition method is typically used in analytical chemistry when analyzing samples with unknown concentrations, where a known amount of standard solution is added to the sample to create a series of solutions with different concentrations. This method is particularly useful when the matrix of the sample interferes with other quantitative methods.


What is correct your friend lives in Broad Street or your friend lives at Broad Street?

Your friend lives on Broad Street is the correct form.You would say: Your friend lives in Albany.You would say: Your friend lives at the corner of Broad Street and River Road.


What is the difference between reagent blank and sample blank?

Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.


What would happen to the pigment spots if the chromatogram was left running for a long time?

If the chromatogram is left running for an extended period, the pigment spots may continue to separate as the solvent carries them further along the stationary phase. This could lead to increased resolution between the different pigments, but if left too long, the spots may become too diffuse and potentially merge into one another. Additionally, excessive time might cause the solvent front to reach the end of the chromatogram, resulting in a loss of resolution and clarity.


When would random sampling not be the best approach to sample selection?

When would random sampling not be the best approach to sample selection


What effect does sample size have on the estimate of the mean?

the larger you r sample size the better your estimate. imagine take the height of person to estimate the average high of an adult male. would one person's height be a good estimate, or would taking the average height of 100, or 5000 adult males will produce a better estimate?