3,5-dinitrosalicylic acid (DNSA) reacts with sugars to form 3-amino-5-nitrosalicylic acid. This is an important reaction because the product (3-amino-5-nitrosalicylic acid) absorbs light at a very specific wavelength (540 nm) which allows for a very accurate quantification of the amount of sugar reacted. For this reason it is used in many medical and forensic testing.
DNSA reagent appears yellow due to ts nitro gp.(chromophore) an alkaline solution of Dnsa is boiled with the reducing sugar to bbe estimated Orange brown coloration result which is read colorimetrically.
by comparing the colours or the amount of precipitate
It detects reducing sugars. DNSA -----> 3-A, 5-N SA
3, 5- dinitrosalicylic acid is the full form of dnsa.
The principle of the DNSA method, which stands for DNA, RNA and protein simultaneous analysis, is to simultaneously analyze DNA, RNA, and proteins in the same sample to provide a comprehensive understanding of molecular interactions and biological processes. This multi-omics approach allows researchers to study how genetic information is translated into functional proteins, enabling a more holistic view of cellular processes.
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I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?