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3,5-dinitrosalicylic acid (DNSA) reacts with sugars to form 3-amino-5-nitrosalicylic acid. This is an important reaction because the product (3-amino-5-nitrosalicylic acid) absorbs light at a very specific wavelength (540 nm) which allows for a very accurate quantification of the amount of sugar reacted. For this reason it is used in many medical and forensic testing.

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Estimation of sugars by DNSA method?

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Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?