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What is role of maskin protein?
Maskin protein prevents eukaryotic mRNAs from being recruited to the ribosome prior to egg fertilization. In other words, maskin halts translation of certain mRNA until after fertilization. In the 3' end of the mRNA there is an Untranslated Region (UTR) (often a rich A region) that does not code for amino acids of the protein. Instead regions of the UTR are binding sites for Cytoplasmic Poly A Element-Binding Protein (CPEB), which binds to maskin. (Other translation inhibitors have similar mechanisms.) This interaction acts to prevent assembly of translation of the mRNA initiation complex until after fertilization. Upon fertilization, phosphorylation of CPEB causes it to undergo a conformational alteration and release from the UTR. Translation can then proceed. Poly(A) binding protein (PBAP) is able to bind to the now open A-rich UTR. PBAP binds to the initiation factor eIF-4G, which is bound to eIF-4E, another initiation factor bound to the 5' cap on the 5' end of mRNA. This binding forms the characteristic loop structure of eukaryotic protein synthesis. Sources: Karp, Gerald C. Cell and Molecular Biology: Concepts and Experiments. edition 5 See the related link for further information.
What is the function of a 5' cap in DNA transcription and translation?
The 5' cap attached to the mRNA ensures the mRNA's stability while it undergoes translation.
What is mRNA base sequence for ATT?
The DNA segment 3' ATT 5' would be transcribed to the mRNA sequence 5' UAA 3'.
What codon on mrna would match this anticodon?
To determine the codon on mRNA that matches a given anticodon, you need to know the complementary base pairing rules. Anticodons are found on tRNA and are complementary to the mRNA codons. For example, if the anticodon is 3'-AUC-5', the corresponding mRNA codon would be 5'-UAG-3'.
What process happens right after transcription ends?
After transcription ends, the newly synthesized mRNA undergoes several processing steps before it can be used for translation. This includes the addition of a 5' cap and a poly-A tail at the 3' end, which protect the mRNA from degradation and aid in its export from the nucleus. Additionally, introns are spliced out, and exons are joined together to form a mature mRNA molecule that can be translated into protein.
Where do Ribosomes bind on mRNA?
Ribosomes bind to the 5' untranslated region (5' UTR) of mRNA, specifically at a sequence called the ribosome-binding site (RBS) or Shine-Dalgarno sequence in prokaryotes. This interaction helps initiate translation by positioning the ribosome at the start codon.
What is role of maskin protein?
Maskin protein prevents eukaryotic mRNAs from being recruited to the ribosome prior to egg fertilization. In other words, maskin halts translation of certain mRNA until after fertilization. In the 3' end of the mRNA there is an Untranslated Region (UTR) (often a rich A region) that does not code for amino acids of the protein. Instead regions of the UTR are binding sites for Cytoplasmic Poly A Element-Binding Protein (CPEB), which binds to maskin. (Other translation inhibitors have similar mechanisms.) This interaction acts to prevent assembly of translation of the mRNA initiation complex until after fertilization. Upon fertilization, phosphorylation of CPEB causes it to undergo a conformational alteration and release from the UTR. Translation can then proceed. Poly(A) binding protein (PBAP) is able to bind to the now open A-rich UTR. PBAP binds to the initiation factor eIF-4G, which is bound to eIF-4E, another initiation factor bound to the 5' cap on the 5' end of mRNA. This binding forms the characteristic loop structure of eukaryotic protein synthesis. Sources: Karp, Gerald C. Cell and Molecular Biology: Concepts and Experiments. edition 5 See the related link for further information.
In which direction is mRNA transcribed?
mRNA is transcribed in the 5' to 3' direction.
What does the 5' junction in mRNA stand for?
The 5' junction in mRNA refers to the point where the mRNA starts, represented by the 5' end of the molecule. This end contains a 5' cap structure that helps protect the mRNA and is important for translation initiation in protein synthesis.
What is the function of the 5' UTR promoter in gene expression regulation?
The 5' UTR promoter in gene expression regulation helps to initiate the process of transcription, which is the first step in making a protein from a gene. It signals the start of transcription and helps regulate the amount of protein that is produced from that gene.
The 5' cap of mRNA is important for what?
The 5' cap of mRNA is important for several reasons: it protects the mRNA from degradation by exonucleases, helps in the recognition and binding of the mRNA by the ribosome for translation, and is essential for efficient splicing of introns.
What is the directionality of mRNA synthesis, specifically in relation to the 5' to 3' orientation?
mRNA synthesis occurs in the 5' to 3' direction, meaning that nucleotides are added to the growing mRNA strand starting from the 5' end and extending towards the 3' end.
What is the function of a 5' cap in DNA transcription and translation?
The 5' cap attached to the mRNA ensures the mRNA's stability while it undergoes translation.
What is mRNA base sequence for ATT?
The DNA segment 3' ATT 5' would be transcribed to the mRNA sequence 5' UAA 3'.
Is mRNA translated from 5' to 3' during the process of protein synthesis?
Yes, mRNA is translated from 5' to 3' during the process of protein synthesis.
What is the directionality of mRNA transcribed from a DNA template, specifically in terms of the 5' to 3' orientation?
mRNA is transcribed in a 5' to 3' direction from a DNA template.
How does the eukaryotic initiation complex locate the true start codon?
The eukaryotic initiation complex locates the true start codon by scanning the mRNA molecule from the 5' cap, aided by initiation factors. The complex recognizes the start codon (usually AUG) in a favorable sequence context and assembles around it to initiate translation.
