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Why you use o-toluidine for glucose estimation?
In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.
HOW TO SEPARATE A MIXTURE OF P-TOLUIC ACID AND P-TOLUIDINE?
To separate a mixture of p-toluic acid and p-toluidine, you can utilize their differing solubilities in an aqueous acid solution. First, dissolve the mixture in a suitable solvent, then add dilute hydrochloric acid; p-toluidine will protonate and dissolve in the aqueous layer as its hydrochloride salt, while p-toluic acid will remain in the organic layer. After separation, you can recover p-toluidine by basifying the aqueous solution, causing it to precipitate out. Finally, p-toluic acid can be isolated by evaporating the organic solvent.
What is a common stain used for plant tissue?
One common stain used for plant tissue is safranin, which is often used to stain plant cell walls and lignified tissues red. There are other stains like toluidine blue and crystal violet that are also commonly used for plant tissue staining.
What dyes are used to stain ribosomes?
Ribosomes can be stained using various dyes, with the most common being methylene blue, which binds to RNA-rich regions. Other dyes include acridine orange and toluidine blue O, which also target nucleic acids. These stains help visualize ribosomes under a microscope, as they highlight the dense RNA content within these cellular structures.
What is the use of acetal?
dilute ethanoic acid is used as a preservative in the preparation of pickles.it is used for making cellulose acetate which is an important artificial fibre.it is used in the manufacture of acetone and esters used in perfumes.it is used in the preparation of dyes.it is used to coaggulate rubber from latex.it is used for making white lead which is used as a white paint.it is used as a chemical reagent in chemistry lab.
Why o-toluidine method cannot be used for the detection of fructose in a solution?
The o-toluidine method is specific to detecting glucose because o-toluidine reacts specifically with aldehyde functional groups on glucose molecules. Fructose does not have an aldehyde functional group, so it will not react with o-toluidine in the same way as glucose, making this method ineffective for detecting fructose in a solution.
Compare the basicity of isomers of toluidine?
As expected from Inductive(/hypercojugative) as well as ortho effect the basicity order should be: ortho-toluidine>para-toluidine>meta-toluidine. But, experimental order is: para-toluidine(Pka=5.12)>meta-toluidine(Pka=4.69)>ortho-toluidine(Pka=4.39).The order of basicity is explained by two opposing effects :i) inductive effect ii) Stability of the conjugate base. Here 2nd factor is more significant for ortho-toluidine & 1st factor is important for para-toluidine. For ortho-toluidine the conjugate base [o-MeC6H4NH3+] is destabilized by steric interaction between -NH3+ & -Me(at ortho). This desatabilization is absent with para-toluidine & meta-toluidine.Tha's why basicity of: ortho-toluidine(Pka=4.39)
Why you use o-toluidine for glucose estimation?
In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.
What is the sensitivity and specificity of ortho toluidine test?
A
Is p-toluidine a solid or a liquid at room temperature?
Para toluidine appears as white lustrous plates or leaflets with an amine odour at room temperature. It melts at 430C.
Is o-toluidine soluble in NaOH?
no it is not. the molecule does not react with the (polar) water molecule, and when NaOH is added the Na and OH dont react with NH2 either (a major functional group on the toluidine molecule) it is not soluble in water either, but when acid is added the NH2 group becomes NH3 and (due to its ionic nature) dissolves.
Why don't we simply oxidize p-toluidine with KMnO4?
Oxidizing p-toluidine with KMnO4 may not be effective because p-toluidine is a primary aromatic amine, which can form a stable complex with KMnO4 and hinder the oxidation process. Additionally, the reaction may lead to side products or incomplete oxidation due to the complex formation.
Is p-toluidine acidic or basic?
P-toluidine is a weakly basic compound due to the presence of an amino group. In solution, it can accept protons to form a positively charged species, making it basic.
Is p-toluidine soluble in HCl?
When I performed this experiment, it did not dissolve. p-toluidine is a very stable molecule, it is also non-polar due to its benzene ring structure. I have a phD in chemistry and have done many similar experiments Dr.Dicks When I performed ths experiment, it dissolved. Since p-toluidine has a basic functional group, it dissolves in the strong acid, HCl. Dr. Horton Ab Ripper X everyday, keeps the doctors away! Unless you get a cardial failure or asthma of course
Biological stains used for plants?
Common biological stains used for plant studies include iodine solution for starch detection, safranin for staining cell walls and nuclei, and toluidine blue for highlighting lignified cell walls. These stains help researchers visualize and study cellular structures and processes in plants.
HOW TO SEPARATE A MIXTURE OF P-TOLUIC ACID AND P-TOLUIDINE?
To separate a mixture of p-toluic acid and p-toluidine, you can utilize their differing solubilities in an aqueous acid solution. First, dissolve the mixture in a suitable solvent, then add dilute hydrochloric acid; p-toluidine will protonate and dissolve in the aqueous layer as its hydrochloride salt, while p-toluic acid will remain in the organic layer. After separation, you can recover p-toluidine by basifying the aqueous solution, causing it to precipitate out. Finally, p-toluic acid can be isolated by evaporating the organic solvent.
Is p-toluidine and organic compound?
Yes, it contains C and H atoms, as well as the organic NH2 functional group.
