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The first stain is crystal violet, next iodine is added which acts as a mordent. Then, alcohol is added which will wash away the purple color from any gram negative bacteria. The purple color will stay in the gram positive bacteria due to their thich peptidoglycan layer. Lastly, Safranin is added as a counter stain. Gram - is red Gram + is purple

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Why is Gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


What is the primary stain used in Gram staining?

The primary stain used in Gram staining is crystal violet.


What method is used to distinguish the two types of bacteria?

One common method to distinguish between different types of bacteria is Gram staining. This method involves staining bacteria with crystal violet and iodine, followed by a decolorization step and counterstain. Based on whether bacteria retain the stain (Gram-positive) or not (Gram-negative), they can be differentiated.


What mordant is used in the process of gram staining?

The mordant used in the process of gram staining is called crystal violet.


Why is safrinin used in gram staining?

as a couterstain


What counter stain is used in gram staining?

safranin


Why gram staining is classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why do gram positive bacteria stain purple in the Gram staining technique?

Gram positive bacteria stain purple in the Gram staining technique because they have a thick layer of peptidoglycan in their cell walls, which retains the crystal violet dye used in the staining process.


Is gram staining related to flagella?

No, gram staining and flagella are not directly related. Gram staining is a technique used to classify bacteria based on cell wall characteristics, while flagella are thread-like appendages that help bacteria move. Flagella presence or absence does not affect the results of a gram stain.


What is the name of the paper used to dry slides after gram staining?

Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.


Is iodine a basic stain?

No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.