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Glucose broth media is primarily used in microbiology to cultivate and support the growth of bacteria, particularly those that utilize glucose as a primary carbon source. It provides essential nutrients and a suitable environment for microbial proliferation and is often employed in fermentation studies, microbial assays, and determining the metabolic capabilities of various organisms. Additionally, glucose broth can be used for the detection of specific bacteria based on their fermentation patterns.

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Why in presumptive test only lactose broth is used not glucose broth?

In presumptive tests for coliforms, lactose broth is preferred over glucose broth because many coliforms can ferment lactose, producing gas and acid, which are indicators of their presence. While glucose can also be fermented by some bacteria, it is less specific for coliforms. The use of lactose broth helps ensure that the test is more selective for lactose-fermenting organisms, making it a better choice for detecting coliforms in water and food samples.


Does Bacillus subtilis grow in MacConkey broth?

Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.


What kind of media is urea broth?

Liquid that comes out of your scrotum when popped.


What reaction would you predict from an organism growing in litmus milk that has the following results in other media?

Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue


Definition of Chemically defined media?

Chemically defined media is a type of growth medium in microbiology containing known quantities of specific chemical components. This allows for precise control over the nutrients available to the microorganisms being cultured, facilitating reproducibility and predictability in experiments. It does not contain complex organic materials such as yeast extract or peptones.

Related Questions

What does SOC stand for in SOC broth?

It stands for 'Super Optimal Broth' for SOB media. The C stands for "catabolite-repression" which indicates that glucose has been added to the media. SOB is just SOC media with that addition.


How are acids detected in a culture medium?

use glucose phosphate broth , inoculate the organism in that broth and incubate at 37 degree celsius for 24 hrs.


What kind of media must one use to determine chitinolytic activity?

Nutrient broth


Why in presumptive test only lactose broth is used not glucose broth?

In presumptive tests for coliforms, lactose broth is preferred over glucose broth because many coliforms can ferment lactose, producing gas and acid, which are indicators of their presence. While glucose can also be fermented by some bacteria, it is less specific for coliforms. The use of lactose broth helps ensure that the test is more selective for lactose-fermenting organisms, making it a better choice for detecting coliforms in water and food samples.


If glucose broth medium is used without a pH indicator how can you determine whether a particular bacterium fermented glucose?

you can use a durham tube and observe the formation of a bubble for the microbes that are expected to produce gas


Does Bacillus subtilis grow in MacConkey broth?

Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.


Is tryptone broth differential or selective media?

Tryptone broth is neither differential nor selective media. It is a nutrient-rich medium used for the growth of a wide range of microorganisms.


What kind of media is urea broth?

Liquid that comes out of your scrotum when popped.


What is the growth in broth media for proteus vulgaris?

hydrogen suifide i think


How do you use broth in a sentence?

i want chicken broth


What reaction would you predict from an organism growing in litmus milk that has the following results in other media?

Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue


What media used to maintain microbes for long periods?

by subculture into non selective media such as soya broth or brain heart