EcoR1 is a restriction enzyme (endonuclease), which splits the phosphodiester bonds of the backbone of DNA.
EcoR1 cuts double-stranded DNA at specific recognition sites generating two fragments, so to generate 4 fragments, EcoR1 would need to cut the DNA twice.
EcoR1 creates sticky ends with a sequence of 5'-GAATTC-3'. This results in protruding ends with a 5' overhang on both strands of the DNA.
The type of molecule that is an enzyme is a protein molecule.
It would be easier for DNA ligase to reconnect two fragments cut by EcoR1, as both fragments would have compatible overhangs that can anneal together. In the case of one fragment cut by EcoR1 and one cut by HindIII, the overhangs produced by the two enzymes are incompatible, making it more challenging for DNA ligase to join them together.
A protein.
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
EcoR1 cuts double-stranded DNA at specific recognition sites generating two fragments, so to generate 4 fragments, EcoR1 would need to cut the DNA twice.
EcoR1 creates sticky ends with a sequence of 5'-GAATTC-3'. This results in protruding ends with a 5' overhang on both strands of the DNA.
A polymer molecule is a macromolecule.
The EcoR1 cut can disrupt the process of DNA replication by cleaving the DNA at specific sites, potentially causing errors in the replication process. This can lead to mutations or changes in the genetic information being copied.
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
The type of molecule that is an enzyme is a protein molecule.
cellulose
It would be easier for DNA ligase to reconnect two fragments cut by EcoR1, as both fragments would have compatible overhangs that can anneal together. In the case of one fragment cut by EcoR1 and one cut by HindIII, the overhangs produced by the two enzymes are incompatible, making it more challenging for DNA ligase to join them together.
The molecule produced after translation of RNA is protein.
A protein.
A hydrogen molecule consists of two hydrogen atoms.