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EcoR1 is a restriction enzyme (endonuclease), which splits the phosphodiester bonds of the backbone of DNA.

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15y ago

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Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?

The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.


How many times EcoR1 cuts the DNA to generate 4 fragment?

EcoR1 cuts double-stranded DNA at specific recognition sites generating two fragments, so to generate 4 fragments, EcoR1 would need to cut the DNA twice.


What sticky ends does EcoR1 create?

EcoR1 creates sticky ends with a sequence of 5'-GAATTC-3'. This results in protruding ends with a 5' overhang on both strands of the DNA.


What kind of molecule is a polymer?

A polymer molecule is a macromolecule.


How does the ecor1 cut affect the process of DNA replication?

The EcoR1 cut can disrupt the process of DNA replication by cleaving the DNA at specific sites, potentially causing errors in the replication process. This can lead to mutations or changes in the genetic information being copied.


What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?

If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.


What kind of molecule is an enzyme-?

The type of molecule that is an enzyme is a protein molecule.


What kind of molecule is formed when many disaccharide molecule's are combined?

cellulose


Would it be easier for DNA ligase to reconnect two fragments cut by EcoR1 or one fragment cut by EcoR1 with one cut by Hind111?

It would be easier for DNA ligase to reconnect two fragments cut by EcoR1, as both fragments would have compatible overhangs that can anneal together. In the case of one fragment cut by EcoR1 and one cut by HindIII, the overhangs produced by the two enzymes are incompatible, making it more challenging for DNA ligase to join them together.


What kind of molecule does translation produce?

The molecule produced after translation of RNA is protein.


What kind of molecule is an enzynme?

A protein.


What kind of atoms are in a molecule of hydrogen?

A hydrogen molecule consists of two hydrogen atoms.