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What would happen if electrodes were plugged into wrong outlets during gel electrophoresis?

If electrodes are plugged into the wrong outlets during gel electrophoresis, the electric current would reverse direction. This would cause the DNA or RNA samples to migrate toward the negative electrode instead of the positive one, resulting in improper separation and potentially causing the samples to run off the gel or not migrate at all. The experiment would yield inaccurate results, making it difficult to analyze the size or quantity of the nucleic acids.


If the electrodes were reversed on electrophoresis?

If the electrodes were reversed on electrophoresis, the negatively charged molecules would move towards the positive electrode and positively charged molecules would move towards the negative electrode. This would result in the opposite direction of separation compared to the intended setup, potentially leading to inaccurate analysis or interpretation of the results.


What would happen to translation if a ribosome skipped one nucleotide?

If a ribosome skipped one nucleotide during translation, it would result in a frameshift mutation. This would alter the reading frame of the mRNA sequence, leading to a nonfunctional or altered protein being produced. Frameshift mutations often have serious consequences as they can disrupt the normal functioning of the protein.


DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?

DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.


When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?

Isolation methods, such as agarose gel electrophoresis, allow visual observation of DNA fragments. Large pieces of genomic DNA will migrate more slowly through the gel compared to small fragments, resulting in distinct bands when stained and viewed under UV light. By comparing the migration of the isolated DNA to molecular weight markers, one can confirm the presence of large DNA fragments.

Related Questions

What would happen if cytokensis was skipped?

If cytokinesis was skipped, the cell would end up with multiple nuclei enclosed within a shared cytoplasm, resulting in a multinucleated cell, a condition known as a syncytium. This would likely lead to impaired cellular function as the cell would be unable to properly segregate genetic material and organelles required for normal cell division and function.


Which type of evidence would be the most appropriate for electrophoresis analysis?

Blood


What would altered conditions in electrophoresis do?

who was the first astronomer to see mountains on the moom


What would happen if electrodes were plugged into wrong outlets during gel electrophoresis?

If electrodes are plugged into the wrong outlets during gel electrophoresis, the electric current would reverse direction. This would cause the DNA or RNA samples to migrate toward the negative electrode instead of the positive one, resulting in improper separation and potentially causing the samples to run off the gel or not migrate at all. The experiment would yield inaccurate results, making it difficult to analyze the size or quantity of the nucleic acids.


Discuss how each what factors would affect the results of electrophoresis?

A person will not be able to discuss how the following factors would affect the results of electrophoresis unless they know what the following factors are. This information needs to be included for a person to know the correct answer.


How would the reversal of polarity effect electrophoresis?

Electrophoresis is the motion of particles relative to some fluid influenced by an electric field. The voltage used will affect this electric field, and in turn affect the movement of particles.


How would the liver maintain homeostasis if a person skipped her lunch?

If a person skipped a meal and the blood sugar levels dropped, the liver could release some of the stored sugar back into the blood.


Which words would form if the prefixes were added to gram?

Some examples are radiogram, telegram, attogram, diagram, pentagram.


How does gel electrophoresis is used to make a DNA fingerprint?

Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.


If the electrodes were reversed on electrophoresis?

If the electrodes were reversed on electrophoresis, the negatively charged molecules would move towards the positive electrode and positively charged molecules would move towards the negative electrode. This would result in the opposite direction of separation compared to the intended setup, potentially leading to inaccurate analysis or interpretation of the results.


What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?

They would be the same since Dolly is clone.


Can gel electrophoresis be used to determine the purity of an enzyme?

Gel electrophoresis can be used to assess the purity of an enzyme by separating different proteins based on size. If the enzyme appears as a single band on the gel, it suggests high purity. Contaminants or impurities would result in additional bands on the gel.