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What else can I help you with?
What condenser value do you want when using 100X objective lens?
When using a 100X objective lens, you typically want to use a high numerical aperture (NA) condenser lens to match the high NA of the objective lens. A condenser with a NA value equal to or greater than the NA of the objective lens (typically around 1.4) is recommended for optimal resolution and contrast in microscopy.
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
When should you use objective lens?
A sample should be examined with low power first when observing any sample for the first time under the microscope. Now if you want to focus on one particular region in the sample then you can step it up to high power lens. Objective lens are not that expensive and good way to look at some simple tissue structures.
Why would you always use the low-power objective lens to locate objects mounted on the slide first even if you want to observe them with the high power objective lens?
because it allows you to find the part of the slide you want to see, then you can zoom in by changing focus to see the frame in greater detail. if you don't start in low power it is extremely difficult to move around the slide
Which objective lens should always be in place when beginning to use the microscope and when putting it away?
The objective lens with the lowest magnification, usually 4x. Be sure to clean all the lens with lens paper before putting the microscope away.
Why is it important to use the fine adjustment knob when using the highest power objective lens?
So you do not crack the high power objective lens - this lens is very fragile and expensive.
If you use the coarse-adjustment knob to focus on an object with the high-power objective what problems will you encounter why?
Using the coarse-adjustment knob with the high-power objective can result in the objective lens getting too close to the slide, potentially damaging the lens or slide. This can also lead to the objective lens hitting the slide, causing a loss of focus and potential damage to both the lens and the slide.
How would you estimate the size of object viewed under high power objective lens 40 times if you were given the size of the field diameter when using low power objective lens 4 times?
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
Why should you only use the fine adjustment knob when you are using high power?
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
6 How does the procedure for using the microscope differ under high power?
For low power you can use the coarse adjustment and fine adjustment for high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Why should the course-adjustment knob on a microscope not be used with a high-power objective lens?
The course-adjustment knob moves the stage up and down quickly, and using it with a high-power objective can potentially damage the objective or slide due to a rapid change in focus. It is best to use the fine-adjustment knob when using a high-power lens for precise focusing.
How does the procedure for using the microscope differ under high power as opposed to low power?
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
How does the procedure for using the microscope different under high power as oppose to low power?
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
Why should you use fine adjust when the high power objective is in position?
So you don't crack the slide or damage the lens
Why a microscope should be stored in low power objective lens?
Storing a microscope with the low power objective lens in place helps protect the lens and prevents accidental damage or contamination during storage. The low power lens is less delicate compared to other high-power lenses, making it a safer option for storage to ensure the microscope remains in good condition for future use.
Which microscope knob do you not use on high power?
You do not use the coarse focus knob on high power because it can damage the slide and the objective lens of the microscope. Use only the fine focus knob to bring the specimen into sharp focus on high power.
