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Why are you allowed to use the coarse adjustment when you focus the low-power objective lens but not when you focus the high power objective lens?
You can use the coarse adjustment knob with the low-power objective lens because it provides a wider field of view and greater depth of focus, making it easier to bring the specimen into general focus. However, using the coarse adjustment with the high-power objective lens risks crashing the lens into the slide, which can damage both the lens and the specimen. Therefore, fine focusing is necessary at higher magnifications to achieve precise focus without risking damage.
When using any high power objective you should not adjust the?
When using any high power objective, you should not adjust the coarse focus knob. This is because the high power objective lens is positioned very close to the slide, and adjusting the coarse focus can lead to crashing the lens into the slide, potentially damaging both the lens and the specimen. Instead, use the fine focus knob for precise adjustments to bring the specimen into sharp focus.
What condenser value do you want when using 100X objective lens?
When using a 100X objective lens, you typically want to use a high numerical aperture (NA) condenser lens to match the high NA of the objective lens. A condenser with a NA value equal to or greater than the NA of the objective lens (typically around 1.4) is recommended for optimal resolution and contrast in microscopy.
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
When should you use objective lens?
A sample should be examined with low power first when observing any sample for the first time under the microscope. Now if you want to focus on one particular region in the sample then you can step it up to high power lens. Objective lens are not that expensive and good way to look at some simple tissue structures.
Why is it important to use the fine adjustment knob when using the highest power objective lens?
So you do not crack the high power objective lens - this lens is very fragile and expensive.
If you use the coarse-adjustment knob to focus on an object with the high-power objective what problems will you encounter why?
Using the coarse-adjustment knob with the high-power objective can result in the objective lens getting too close to the slide, potentially damaging the lens or slide. This can also lead to the objective lens hitting the slide, causing a loss of focus and potential damage to both the lens and the slide.
How would you estimate the size of object viewed under high power objective lens 40 times if you were given the size of the field diameter when using low power objective lens 4 times?
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
Why are you allowed to use the coarse adjustment when you focus the low-power objective lens but not when you focus the high power objective lens?
You can use the coarse adjustment knob with the low-power objective lens because it provides a wider field of view and greater depth of focus, making it easier to bring the specimen into general focus. However, using the coarse adjustment with the high-power objective lens risks crashing the lens into the slide, which can damage both the lens and the specimen. Therefore, fine focusing is necessary at higher magnifications to achieve precise focus without risking damage.
Why should you only use the fine adjustment knob when you are using high power?
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
6 How does the procedure for using the microscope differ under high power?
For low power you can use the coarse adjustment and fine adjustment for high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Why should the course-adjustment knob on a microscope not be used with a high-power objective lens?
The course-adjustment knob moves the stage up and down quickly, and using it with a high-power objective can potentially damage the objective or slide due to a rapid change in focus. It is best to use the fine-adjustment knob when using a high-power lens for precise focusing.
When focusing an image under high power you should use the?
When focusing an image under high power, you should use the fine focus knob. This allows for precise adjustments and helps avoid damaging the slide or the objective lens. Additionally, start with the lowest power objective lens to locate the specimen before switching to high power for detailed observation. Always ensure the stage is lowered before switching to high power to prevent contact between the lens and the slide.
How does the procedure for using the microscope differ under high power as opposed to low power?
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
How does the procedure for using the microscope different under high power as oppose to low power?
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
Why a microscope should be stored in low power objective lens?
Storing a microscope with the low power objective lens in place helps protect the lens and prevents accidental damage or contamination during storage. The low power lens is less delicate compared to other high-power lenses, making it a safer option for storage to ensure the microscope remains in good condition for future use.
