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When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?
Isolation methods, such as agarose gel electrophoresis, allow visual observation of DNA fragments. Large pieces of genomic DNA will migrate more slowly through the gel compared to small fragments, resulting in distinct bands when stained and viewed under UV light. By comparing the migration of the isolated DNA to molecular weight markers, one can confirm the presence of large DNA fragments.
If you wanted to clone a sequence of DNA - 200 kb long - what vector should you use?
For cloning a 200 kb DNA sequence, a bacterial artificial chromosome (BAC) vector would be suitable due to its large insert capacity, stability, and ability to maintain large DNA fragments intact. BAC vectors can accommodate DNA inserts up to 300 kb in size, making them ideal for cloning large DNA fragments accurately.
Why is the largest DNA fragment band found closest to the well in which it was placed?
The largest DNA fragments travel more slowly through the agarose gel due to their size, so they don't move as far from the well as smaller fragments during gel electrophoresis. This results in the largest fragments being closest to the well after electrophoresis is completed.
Okazaki fragments are joined together by?
During DNA replication Okazaki fragments are joined together by DNA polymerase. Remember that Okazaki fragments start with an RNA primer so RNAse H is need to remove the primer follwed by DNA plymerase to add nucleotides and finally DNA ligase to seal the single strand nick.
What stand of DNA replicated in okasaki fragments?
The lagging strand of DNA is replicated in Okazaki fragments. These short, discontinuous fragments are synthesized as the DNA replication process moves away from the replication fork. They are eventually joined together by DNA ligase to form a continuous strand.
When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?
Isolation methods, such as agarose gel electrophoresis, allow visual observation of DNA fragments. Large pieces of genomic DNA will migrate more slowly through the gel compared to small fragments, resulting in distinct bands when stained and viewed under UV light. By comparing the migration of the isolated DNA to molecular weight markers, one can confirm the presence of large DNA fragments.
The rate at which large DNA fragments move through the electrophoretic gel is?
The rate at which large DNA fragments move through the electrophoretic gel is slower compared to small DNA fragments because larger fragments experience more resistance as they navigate through the gel matrix. This results in larger DNA fragments being located closer to the well where they were loaded onto the gel, while smaller fragments move further down the gel towards the positive electrode.
What does Taping the DNA onto large paper simulate?
Taping DNA onto large paper simulates DNA electrophoresis, a process used to separate and visualize DNA fragments based on size. By laying out the DNA fragments in a straight line, it allows for easier analysis and comparison of different DNA samples.
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
If you wanted to clone a sequence of DNA - 200 kb long - what vector should you use?
For cloning a 200 kb DNA sequence, a bacterial artificial chromosome (BAC) vector would be suitable due to its large insert capacity, stability, and ability to maintain large DNA fragments intact. BAC vectors can accommodate DNA inserts up to 300 kb in size, making them ideal for cloning large DNA fragments accurately.
How do you get DNA fragments in Bakugan dimensions?
You get DNA fragments by entering Bakugan codes.
What is the process of adding fragments of DNA to other DNA called?
The process of adding fragments of DNA to other DNA is called DNA ligation. This involves joining together two DNA fragments using an enzyme called DNA ligase, which helps to form a covalent bond between the DNA fragments.
What is the function of a DNA ladder in gel electrophoresis and how does it aid in the analysis of DNA fragments?
In gel electrophoresis, a DNA ladder serves as a reference for determining the sizes of DNA fragments being analyzed. It contains DNA fragments of known sizes, which help in estimating the sizes of unknown DNA fragments by comparison. This aids in accurately identifying and analyzing the DNA fragments present in the sample.
What do you do with DNA fragments in bakugan dimensions?
When You collect 20 DNA fragments you get a free bakugan
What are DNA fragments that have attached RNA fragments called?
Okazaki fragments.
Did reiji okazaki invent anything?
Technically he did not "invent" anything, but he found out and proved that one of the two strands in new forming DNA during replication is replicating discontinuously, which means there are small fragments of DNA, which are to be connected afterwards. These fragments are named after him Okazaki fragments.
What are the fragments making up the noncontinuous strand called?
The fragments making up the noncontinuous strand in DNA replication are called Okazaki fragments. These are short DNA fragments that are synthesized discontinuously on the lagging strand during DNA replication.
