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Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
What enzyme assembles the complimentary nucleotide bases during replication?
THat would be the enzyme DNA Polymerase III which attaches free floating nucleotides to the parent strand. But remember, they can only be attached to a free 3' position!
Which protein would you need to complete the synthesis of a new strand of DNA?
You would need a DNA polymerase protein to complete the synthesis of a new strand of DNA. DNA polymerase is an enzyme that assembles new DNA strands by adding nucleotides one by one in the 5' to 3' direction.
If a cell lacked the enzyme RNA polymerase it could not what?
If a cell lacked the enzyme RNA polymerase, it could not synthesize RNA from a DNA template, which is essential for the process of transcription. This would prevent the cell from producing messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA), thereby disrupting protein synthesis and various cellular functions. Ultimately, the absence of RNA polymerase would hinder gene expression and the overall viability of the cell.
RNA polymerases are transcripted by which enzyme?
You seem confused. RNA polymerase is the enzyme that transcribes DNA into pre mRNA. So, the enzyme would transcribe the messenger RNA for its own protein construction.
Which enzyme would probably not be needed for DNA replication?
DNA helicase
Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
What enzyme assembles the complimentary nucleotide bases during replication?
THat would be the enzyme DNA Polymerase III which attaches free floating nucleotides to the parent strand. But remember, they can only be attached to a free 3' position!
What is the enzyme that synthesized DNA used in PCR that distinguishes it from the equivalent enzyme that carry out the same function in our cells or those of most bacteria?
The enzyme used in PCR to synthesize DNA is called DNA polymerase. The key difference is that the DNA polymerase used in PCR, such as Taq polymerase, is derived from a thermophilic bacterium called Thermus aquaticus and can withstand the high temperatures used in the PCR cycling process. This distinguishes it from the equivalent enzyme in our cells or most bacteria, which would be denatured by the high temperatures of PCR.
Which protein would you need to complete the synthesis of a new strand of DNA?
You would need a DNA polymerase protein to complete the synthesis of a new strand of DNA. DNA polymerase is an enzyme that assembles new DNA strands by adding nucleotides one by one in the 5' to 3' direction.
RNA polymerases are transcripted by which enzyme?
You seem confused. RNA polymerase is the enzyme that transcribes DNA into pre mRNA. So, the enzyme would transcribe the messenger RNA for its own protein construction.
What bonds do helicase and polymerase enzymes break?
Helicase enzyme breaks hydrogen bonds between base pairs in DNA strands to unwind the double helix structure. Polymerase enzyme breaks the bonds between nucleotides in the DNA strand being replicated, allowing for the addition of new nucleotides during DNA replication.
What is the name of the enzyme that would have placed nucleotides into the replicating DNA in the correct order?
The enzyme responsible for placing nucleotides into replicating DNA in the correct order is called DNA polymerase. DNA polymerase adds complementary nucleotides to the growing DNA strand during replication, following the rules of base pairing (A with T and C with G).
What is the importance of RNA Polymerase?
RNA polymerase is essential for gene transcription, as it catalyzes the synthesis of RNA using a DNA template. It plays a key role in gene expression and regulation by transcribing DNA into RNA, which is then translated into proteins. Without RNA polymerase, cells would not be able to produce the necessary proteins for their survival and function.
How would RNA polymerase be affected if the repressor protein were not bound to the proper site on a gene?
If the repressor protein is not bound to the proper site on a gene, it would not block the RNA polymerase from transcribing the gene. This would lead to the expression of the gene, as the RNA polymerase can then proceed with transcription.
What happens to a cell if it lack the enzyme DNA polymerase?
If a cell lacks DNA polymerase, it would not be able to replicate its DNA properly during cell division. This could lead to errors in the DNA sequence, potentially causing mutations and impairing the cell's ability to function correctly. Ultimately, this could result in cell death or contribute to the development of diseases such as cancer.
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
