Chromatography

The stationary phase is the medium that is used to react with the mobile phase. The molbile phase is sent through the column. The stationary phase is inside the column and reacts with your carrier liquid (mobile phase) which contains the sample that you want to be analyzed.

Stationary phase is different for every column because you need different mediums for different tests.

A solution of amino acids is spotted onto a piece of chromatography paper which is then placed into a container filled with a suitable solvent. A dye is used so that the position of the amino acids along the piece of paper can be seen. The distances travelled by the amino acids are measured to calculate their retention factors (Rf) values. These are then compared to known standards.

Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.

Yes, gas chromatography is a highly reliable analytical technique used in various industries such as food, environmental, and pharmaceutical. It offers high sensitivity, precision, and reproducibility in separating and analyzing complex mixtures of compounds. Proper calibration, maintenance, and use of quality standards can further enhance the reliability of gas chromatography results.

different dyes travel with different speed/rates on filter paper in paper chromatography as they have nothing to do with the filter paper as it is the stationary medium while the rates depends upon the interaction between the solute i.e., dyes and the mobile phase i.e., the solvent.

chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...

Rf is about .45

mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill

hydrocarbon

Paper chromatography is used as an analytical method not for production.

two liquids having different solubility are separated by chromography.

Schiff bases are imines formed by the condensation of aldehydes or ketones with primary amines. The mobile phase for this could be ethyl acetate in hexane or chloroform in methanol.

A keeper is a iron or a steel bar that is used to retain the strength of magnet.

The Dionex company manufactures chromatography, ion chromatography, and extraction systems for identifying components of chemical mixtures. Chromatography is the process of separating different kinds of mixtures.

Infrared spectrometry can provide a complex fingerprint which is unique to the compound being examined. As its instruments as computer controlled, the matching of the spectrum of a compound to its standard fingerprint can be readily carried out.

because the pen or marker will run and the pencil will not.Did this answer your question(if this did not you are a complete idiot):)

Chromatography is used to separate mixtures.In many labs chromotography is used to see whether sweets for example contains prohibited dyes.Forensics use this method of separation to deduce substances.In fact your question is vast.You should have specified what you mean by when

I don`t know

Splitless injection is used as the concentration of all gases is low in the human body. Split injection is only used for concentrated samples.

No

Dead time in chromatography refers to the time it takes for an unretained compound, which does not interact with the stationary phase, to travel through the column. It is essentially the time required for the mobile phase to pass through the system without any solute. This parameter is crucial for calculating retention times of analytes and helps in evaluating the efficiency of the chromatographic separation. Understanding dead time aids in optimizing the separation process and improving the resolution of the chromatographic method.

The use of silica gel can be interpreted in two ways. It well know that silica (SiO2) can adsorb water on its surface. If you use silica dryed under 400 °C, the process of adsorption occurs by the silanol group. So, molecules with a polar groups like hydroxil, amine, nitrilic ... can make chemical interactions with the silica surface and can be retained more time into a column. In this case the adsorption is chemical. But, if you use a deactived silica (~5% H2O), the H2O molecules make a layer into a silica surface and the main process occurs by partition chromatography.

thermocline