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Microscopes
Microscopes are used to look at things that are not visible to the naked eye. This category would demonstrate how microscopes work and information about different kinds of microscope.
4,322 Questions
What are the two basic principles involved in using a microscope?
principle of microscopy includes 2 important aspects:
1. resolution and resolving power- the resolving power of a microscope is defined as the minimum distance by which 2 points must be separated in order for them to be perceived as 2 separate points rather than a fused image.
2. magnification- Linear (sometimes called lateral or transverse) magnification refers to the ratio of image length to object length measured in planes that are perpendicular to the optical axis. A negative value of linear magnification denotes an inverted image. Longitudinal
magnification denotes the factor by which an image increases in size, as measured along the optical axis.
What are the differences and similarities between light TEM and SEM microscopes?
a TEM microscope privides an detailed image of the inside of a specimen
a SEM microscope provides a 3D image of a specimen
take for exampel a sperm in a TEM microscope you would see the inner stucture of the sperm
in a SEM microscope you would se in detail the exact form shape of the sperm
What microscope is used to focus under low power only?
Course Focus Knob- low
Fine Focus Knob- high
-MC
What allows the user of a microscope to limit the amount of light from a steady light source?
A diaphragm or iris control on the microscope allows the user to adjust the amount of light reaching the specimen from a steady light source. By opening or closing the diaphragm, the user can control the intensity of the light to optimize visibility without overwhelming the sample.
Can you see atoms with a microscopes?
Traditional light microscopes cannot see individual atoms due to their limited resolution, typically on the scale of hundreds of nanometers. Specialized techniques such as scanning tunneling microscopy and atomic force microscopy have been developed to image individual atoms by scanning a sharp probe tip over a surface at extremely close distances.
When you move the microscope slide towards you, the image appears to move in the opposite direction (away) to maintain focus. Conversely, when you move the slide away from you, the image appears to move closer (towards you) to stay in focus. This is known as the parfocal property of microscopes.
What is the function of the condenser lens on the micrscope?
The condenser lens focuses and directs light onto the specimen on the microscope slide. It helps to illuminate the specimen evenly and improve contrast and resolution for clearer imaging. Adjusting the condenser lens controls the brightness and sharpness of the image.
Where should one hold the microscope?
One should hold the microscope firmly by the arm and the base when carrying it to ensure stability and prevent any damage. Holding it this way also helps in maintaining the balance of the microscope, especially when moving it from one place to another.
Why true solutions are not visible with microscope even with electron microscope?
True solutions contain particles that are smaller than the wavelength of visible light, making them invisible even under a microscope. Electron microscopes use electrons instead of light to visualize objects, but they may still not be able to detect the individual particles in a true solution due to their small size and lack of scattering properties.
What are three ways to keep the microscope from getting damaged?
Always carry a microscope with two hands. Place one hand under the base of the microscope and hold the arm with the other hand. Do not allow the microscope to drop or bump into anything while carrying it. This could damage the microscope or cause small pieces to break off that could cause a tripping hazard. Touching the microscope lens can smudge or damage it. Always use lens paper or a soft cloth with a small amount of isopropyl alcohol to clean a dirty microscope lens. Remove slides and cover slips from the stage before storing the microscope, and rotate the nose piece so that the smallest power objective is facing the stage. Place a dust cover over the microscope when storing it to prevent dust and dirt from damaging the machine. Read more about Microscope Saftey Procedures in the related link.
What is the meaning of course adjustment?
Course adjustment refers to making corrections or changes to plans or actions in order to stay on track towards a goal or objective. It involves assessing current progress, identifying any deviations or obstacles, and then making necessary alterations to ensure successful outcomes. This process helps to maintain flexibility and adaptability in the face of changing circumstances.
Why is 100x microscope lens called oil immersion lens?
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
What mechanical part of the microscope holds the objectives?
The revolving nosepiece, also known as the turret, is the mechanical part of the microscope that holds the objectives. It allows for easy switching between different objective lenses for varying levels of magnification.
What is the meaning of rack and pinion substage in a compound microscope?
The rack and pinion substage in a compound microscope is a mechanism that allows you to adjust the vertical position of the stage. By turning the knob, the rack and pinion system moves the stage up or down to focus the specimen being observed under the objective lens. This feature helps to achieve sharp images by adjusting the distance between the specimen and the objective lens.
What mechanical part of the microscope holds the three objectives?
The revolving nosepiece is the mechanical part of the microscope that holds the three objectives. It allows you to easily switch between different magnifications by rotating the nosepiece to select the desired objective lens.
What is the magnifying power of the LPO?
The magnifying power of the LPO (low power objective) lens on a microscope is typically 10x. This means that when viewing an object through the LPO lens, it will appear 10 times larger than its actual size.
Why is the electron microscope more powerful than the optical microscope?
Short Answer:
Electron microscopes have much better resolution and are capable of much higher magnification than light microscopes because the wavelength of the electrons is thousands of times smaller than the wavelength of light.
Light microscopes can typically resolve structures to a fraction of a micron compared to electron microscopes which in practice achieve resolutions of a few nanometers. Practically, electron microscope can have almost a thousand times greater resolution than an optical microscope.
The useful magnification of an electron microscope is also in the range of a thousand times greater than an optical microscope.
The actual performance of any microscope depends on its design and lensing system and so significant variation exists in the above practical characteristics and performance of both types of microscopes can be enhanced in various ways.
Long Answer:
An electron microscope uses an electron beam to illuminate a specimen and produce the image.
An optical or light microscope uses a light beam to illuminate a specimen and produce the image.
A microscope of either type is characterized by its magnification and resolving power. The magnification depends on the lensing system and can be increased to any degree, but the maximum useful magnification is limited by the resolving power.
The resolving power of a microscope can not be better than the limits placed on it by the size of the wavelength of the illuminating beam. The smaller the wavelength, the smaller the structures that can be resolved in them image.
Visible wavelengths of light are a few hundred nanometers. An electron microscope operates with electrons accelerated to a few hundred thousand electron volts of energy and with a wavelength in the range of few hundredths of an Angstrom.
An electron microscope has a theoretical resolving power that is much greater than a light microscope and can reveal smaller structures because the electrons used have wavelengths (few hundredths of Angstroms) almost 100,000 times shorter than visible light (few hundred nanometers).
An optimized electron microscope can achieve a practical resolution of a few Angstroms and a useful magnifications in the millions of times.
A good light microscope can resolve structures smaller than a micron but is limited to about a few hundred nanometers resolution. The useful magnification of a light microscope is not much more than a thousand times.
The electron microscope uses electrostatic and electromagnetic fields to act as lenses to control and focus the electron beam and to form an image. An optical or light microscope employs glass lenses.
Why is necessary to focus with the fine adjustment in high magnification?
Fine adjustment is necessary in high magnification because it allows for precise focusing on details of the specimen. At high magnifications, even small adjustments can drastically change the focal plane, so fine adjustment helps to bring the object into sharp focus without overshooting. This is essential for obtaining clear and detailed images when using powerful magnification levels.
What are the parts of microscope that are illuminating parts?
a. Eye piece
b. Mirror
c. Diaphragm
d. Pillars
Eye piece- let the observer peeks through
Mirror- one that collects and reflects the lights
Diaphragm- one that regulates the amount of light into the microscope
Pillars- cost that support microscope
How electron microscopes are different from light microscopes?
Electron microscope gives a greater magnification. Instead of lenses, the electron microscope uses magnets to aim a beam of electrons at thin slices of cells.
A light microscope does not have as good as a magnification and resolution as a electron mircroscope does. A microscope as a curved lens, so the light bounces off of it and makes the small object larger.
How does a phase-contrast microscope differ from a bright field microscope?
1.diffrent typeof diaphraghms
2.a phase plate
3.living sample can be viewed
4.natural processes ccan be viewed without staining
5.enhances clarity
6.can be of two types :positive and negative
positive:background much brighter than specimen
negative:background is darker
How do you clean a compound light microscope?
To clean a compound light microscope, start by using a soft, lint-free cloth to gently wipe down the exterior surfaces. Use a can of compressed air to remove dust from the lenses and internal components. For more stubborn dirt or debris, you can use a small amount of lens cleaning solution on a lens tissue to carefully clean the lenses. Avoid using harsh chemicals or abrasive materials that could damage the microscope.
What is the smallest thing that the electron microscope can see?
Electron microscopes use a particle beam of electrons to illuminate the specimen and produce a magnified image with better than 50 pm resolution and magnifications of up to about 10,000,000 times.
What does top it the knob mean?
"Top it the knob" seems to be a typo or misspelling. It does not have a known meaning or commonly used phrase. Can you provide more context?
What part of microscope secure the slide to the stage?
The mechanical stage clips on a microscope secure the slide in place during observation by holding it firmly against the stage.
