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Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular Biology as a way of introducing some substance into a cell, such as loading it with a molecular probe, a drug that can change the cell's function, or a piece of coding DNA.
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What kind of technology is electroporation?
Electroporation is the technology that is connected to the increase of electrical conductivity that is seen in the cell. This can cause the cell membrane to weaken and be more easily punctured.
How electroporation increase permeability of plasma membrane?
Electroporation is a dynamic phenomenon that depends on the local transmembrane voltage at each point on the cell membrane. It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon (from 0.5 V to 1 V). This leads to the definition of an electric field magnitude threshold for electroporation (Eth). That is, only the cells within areas where E≧Eth are electroporated. If a second threshold (Eir) is reached or surpassed, electroporation will compromise the viability of the cells, i.e., irreversible electroporation.[2]
What has the author Jane Edgerton written?
Jane Edgerton has written: 'Electroporation of mammalian cells'
What are the differences between heat shock and electroporation methods for transforming cells?
Heat shock and electroporation are two methods used to transform cells by introducing foreign DNA into them. Heat shock involves briefly exposing cells to high temperatures, which increases their permeability and allows the foreign DNA to enter. Electroporation, on the other hand, uses an electric field to create temporary pores in the cell membrane, through which the foreign DNA can pass. In summary, the main difference between heat shock and electroporation methods is the mechanism by which they make cells more receptive to foreign DNA.
Can E Coli be transformed?
I don`t know other ways but I know that It can be transformed chemically & by electroporation
What are the differences between electroporation and heat shock methods for introducing foreign DNA into cells?
Electroporation and heat shock are two methods used to introduce foreign DNA into cells. Electroporation involves applying an electric field to create temporary pores in the cell membrane, allowing the DNA to enter the cell. Heat shock, on the other hand, involves briefly exposing the cells to high temperatures, which causes the cell membrane to become more permeable, allowing the DNA to enter. In summary, electroporation uses an electric field to create pores in the cell membrane, while heat shock uses high temperatures to make the membrane more permeable.
How are genes transfered?
gene transferring methods includes: gene gun method, biopsy, electroporation, biolistics, cloning vectors.
5 ways of getting DNA into a cell?
Transformation: introducing naked DNA into bacterial cells through heat shock or electroporation. Transduction: using a viral vector to deliver DNA into cells. Conjugation: direct transfer of genetic material between bacterial cells through a conjugative bridge. Lipofection: using lipid-based molecules to facilitate the entry of DNA into eukaryotic cells. Electroporation: applying an electric field to create transient pores in cell membranes for DNA uptake.
How is electropitation done?
No such word in the dictionary or encyclopedia. There is the word electroporation that refers to electrical pulses used to create temporary pores in cell membranes.
Where is it proven that Robert C Beck DSc physics' biocompatible electric current applied to bodily tissues-blood electrification etc is not low-power electroporation-EP similar to drug delivery EP?
(Source- http:/www.toolsforhealing.com/CD/Articles/Q/QuestionsAnswersabouttheB.html - as posted by Michael Forrest on January 11, 1998) From Brian Austin, Technical Support Manager at Genetronics (an electroporation device company), "with the (electrical) conditions use (by Bob Beck's Black Box), there should be no electroporation effects. Field strengths are not high enough." From J. C. Weaver of Massachusetts Institute of Technology in his report on electroporation published in the Journal of Cellular Biochemistry (51:426-435 1993) reported "In the case of isolated cells, mammalian cells experience electroporation for electric fields of about E=1kv/cm (1000 volts with a distance of 1 centimeter between electrodes) for short pulses." The Black Box produces about 1.35 volts per centimeter when the electrodes are placed on the same wrist. From another electroporation device manufacturer, Cyto Pulse Sciences, stated on their website (in the equipment tutorial) that red blood cells need 1430 volts per centimeter for electroporation. However, there is evidence that blood cells are more absoptive during electricfication in the Biophysics Journal, Vol. 58 Oct 1990, pages 897-903 where the authors wrote "...electric fields that generate transmembrane potential in the range of millivolts are capable of activating membrane transport systems." (The Black Box creates a potential across the blood cell membranes of millivolts.) Also;"...reversible activation of certain membrane channels or transport systems may be achieved using low-amplitude, low frequency alternating current fields. Electric fields as small as 16 volts per centimeter have been shown to induce membrane conductance , and these effects were completely reversible. Human erythrocytes treated with such ac fields for hours were shown to have normal shape, volume, and permeabilities to potassium and sodium."
What has the author Ming-Mei Chang written?
Ming-Mei Chang has written: 'Tissue culture and electroporation of potato (Solanum tuberosum L. cv. Russet Burbank' -- subject(s): Potatoes, Genetics
What has the author Vani Akella written?
Vani Akella has written: 'Development of novel techniques of legume transformation using electroporation of intact plant tissue' -- subject(s): Genetics, Plant cells and tissues, Peas, Cowpea
