to calculate signal to noise use this formulae peak hight *2/noise level
what i8 peak to valley ratio in HPLC
mixture of enantiomers can be separated by HPLC
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
HPLC is used to analyze, identify, purify and quantify organic compounds.
A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
HPLC is advanced
why RT was shifting & how to RT calculation in HPLC
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
mixture of enantiomers can be separated by HPLC
"RS-HPLC method" means "Related Substance HPLC Method".
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor