What are the steps of using recombinant DNA technology?

Answer 1

1 Isolate DNA

2 Cut DNA with a restriction enzyme

3 Mix the DNA's and join then together by using DNA ligase

4 Insert the recombinant plasmid into a host bacterium

5 Allow the bacterium to reproduce

Answer 2

The steps of using recombinant DNA technology are:

1. isolation and cutting of DNA of required gene

2. introduce cut DNA into a vector

3. amplification of gene with the help of PCR

4. introduce into compotetant host

5. obtaning gene product

6. down processing

Answer 3

The steps involved in rDNA technology are:

1. Isolation of DNA
2. Fragmentation of the DNA using the enzyme Restriction endonucleases
3. Isolation of the desired DNA fragment
4. Amplification of the gene of interest
5. Ligation of the DNA fragment into a suitable vector by the enzyme DNA ligases
6. Transfer of DNA into the host cell
7. Culturing the host cells on a suitable medium on a large scale
8. Extraction of the desired product
9. Downstream processing of the products

Answer 4

eg insulin
1- isolation of human gene
mRNA is taken from beta cells and used to make DNA with the
help of reverse transscriptase. it is then cute to leave sticky ends.
2-Preparation of vector
plasmids from bacteria are cut with the same restriction enzyme to
produce sticky ends.
3- Formation of recombinant DNA, identification and cloning
the insulin DNA and plasmid DNA are mixed together with ligase
enzyme the sticky end bases form hydrogen bonds. the ligase
joins the DNA backbone and a recomninant plasmid is produced.
- the recombinant plasmid is introduced into bacteria
- a transformed bacterium is identified
- the gene is cloned by the growing bacterium

Answer 5

1.) Find an organism you want to study2.) Isolate its genomic DNA

3.) Find the sequence of DNA you are interested in. A piece of DNA that codes for a protein for example.

4.) Amplifying that DNA using a polymerase chain reaction

5.) Clone into a vector of choice.

All of these you expand into more steps which can be overwhelming if you haven't done this before. These are the big picture steps.

Answer 6

The correct order of steps in doing genetic engineering is cleaving, producing recombinant DNA, cloning, and screening.

Answer 7

1. Gene is taken from organism 1

2. gene is transferred to organism 2

3. organism 2 has new traits

4. New genes are made in organism 1

or... B on plato.

Answer 8

1.cut out a piece of DNA from a DNA molecule.

2.use a restriction enzyme to form sticky ends in DNA.

3.slice a piece of DNA from another organism.

Answer 9

DNA molecules from different sources are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes.