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What is decoy oligonucleotides?
Transcription Factor oligodeoxynucleotide decoys (ODN decoys). Use our GeneDetect® transcription factor decoys to inhibit specific transcription factors in cell culture. Complete list of Transcription Factor decoy Products. Introduction
Transcription factor ODN decoy approach
Advantages and disadvantages of the ODN decoy approach for studying cellular gene expression
ODN Decoys available from GeneDetect.com
How are these decoys used?
Cells can respond to stimuli (normal or pathological) by changing the levels of expression of specific genes. The cellular proteins that regulate changes in gene expression are called transcription factors. Transcription factors are generally nuclear and can either be constitutively expressed within the cell (present under basal conditions, for example CREB) or themselves inducible (for example AP-1). These transcription factor proteins bind specific sequences found in the promoter regions of genes (target genes) whose expression they then regulate (switch on or off). These binding sequences are generally 6-10 base pairs in length and are occasionally found in multiple copies within the promoter regions of target genes. Although the transcription factor protein-DNA interaction is sequence-specific, the binding site for one given transcription factor may vary by several base pairs within different target genes. Therefore when we describe the specific DNA binding sequence for a transcription factor we refer to the non-variable part of the binding sequence, that is, the transcription factor consensus sequence. For example, the AP-1 transcription factor made up of Fos and Jun proteins binds to the TGACTCA consensus sequence. In comparison the consensus sequence for the Smad transcription factor family which mediate TGF-b, activin and BMP induced changes in gene expression is CAGACA. Fig. 1. Transcription factor ODN decoy approach. The basic theory behind the transcription factor ODN decoy approach involves flooding the cell with competing synthetic, transcription factor-specific consensus sequences. These synthetic decoys "compete" for binding of the transcription factor with consensus sequences in target genes. If delivered into the cell in sufficient concentrations these "decoys" thus have the potential to attenuate the binding of the transcription factor to promoter regions of target genes and thus attenuate the function of the transcription factor to regulate the expression of its target gene(s). Transfected at high concentrations these decoys have been reported in the literature to completely block transcription factor function. Clearly they represent powerful research tools for studying gene regulation both in vitro and also more recently in vivo (for Reviews see Moshita et al., 1998, Mann and Dzau, 2000). Fig. 2. Advantages and disadvantages of the ODN decoy approach for studying cellular gene expression. Advantages.
- ODN decoys offer a means of specifically inhibiting transcription factor function in living cells.
- Inexpensive compared to other more classical methods of investigating gene expression such as chloramphenicol acetyltransferase and luciferase constructs in promoter-reporter gene transfection experiments.
- Allows for investigation of both endogenous and pathological gene regulation
- Proven to be highly effective and selective within in vitro experiments.
- Easy to use.
- An emerging technology that has not yet been fully characterized
- Issues of decoy synthesis. High levels of purity and stability required.
- Transfection issues. Which method is best. How to optimize transfection.
- Issue of controls. What controls are needed.
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yes it is. just got back from yuma yesterday and the hunting was great!! the decoy works well. take yours out and have a good time.
A Decoy Detonator is a black horn-like object. When dropped, it runs a reasonable distance away and makes a loud bang noise. It then releases clouds of black smoke. It is used… as a diversion and was invented by Fred and George Weasley. They are sold at Weasleys' Wizard Wheezes.
The amount of line needed depends on where you plan to hunt. The deeper the water, the more line you need. If the weight does not touch the bottom, the weight on the bot…tom will cause your decoy to be pulled partially underwater, which takes away from lifelike qualities and be more likely to drift away. When i tie my decoy strings i have 4 dozen that have short lines roughly 2 1/2 to 3 feet long and also some that range from 10 to 12 feet (conditions that you would not want to wade into, but use a dog or boat).
As of right now, there is no where to buy the studio version of Decoy. Decoy was the exclusive bonus track on the Hot Topic version of Riot!. As it was a Hot Topic exclusive t…rack, it cannot be downloaded on iTunes, Amazon, etc. A live version of Decoy is played on The Final Riot!, which can purchased in CD stores as well as digitally.
For example, when the outside hitter comes behind the middle blocker and hits, the middle blocker will jump up while the outside hitter behind the middle hits the ball. The Mi…ddle blocker acts as a decoy to trick the blockers on the opposing team.
The plural form for the singular noun decoy is decoys. The plural possessive is decoys'. Example: The decoys' realism was amazing.
Yes, presidents do have decoys incase the White House in under attack, or incase some one is trying to assasinate the president.
is a grouping of soiled tablewares in soiled dish table according to size and shape
decoy system is a grouping of soiled tablewares in soiled dish table according to size and shapes.
no there isn't a 3rd decoy's movie.
Yes, just like Marine One (Presidential Helicopter). They use two helicopters for a decoy. Same with Air Force One.
The plural of decoy is decoys.
the plural form of decoy is decoys.
If one is asking if deer decoys are legal to target shoot on then, yes, they are legal to target shoot on and are used for practice with crossbows and compound bows. If …you are asking if they are legal to lure deer one should assume so because in the 2007-2008 season the PA laws have been adapted to let one bait deer using such tactics as salt licks and mineral licks. Seeing as a decoy deer it should be legal because it would be a much more tamed down version of luring deer.
Prior to Operation Overlord, the invasion of Normandy, the Allies ran a deception operation to convince the Germans that the invasion was planned for Calais. The name of this …was Operation Quicksilver. The Allies were aware that the German High Command believed that a major cross Channel invasion required taking a seaport. The Germans did not believe that the Allies could provide enough supplies for the invasion across beaches. Since Calais is the narrowest point of the Channel, it was a logical choice. Operation Quicksilver had several aspects. Thousand of dummy tanks and truck (both inflatable and wood and canvas) which were placed in fields in England opposite Calais. German recon planes were allowed to photograph them. General George Patton was placed in command of the fictional First U.S. Army Group and he made numerous public appearances around Ipswich and Maidstone to fool the Germans into thinking that a huge force was assembling there (in WWII an "army group" was somewhere from 500,000 to 1,500,000 men). The Signal Corps generated thousands of bogus radio messages each day from this area, that the Germans intercepted and interpreted as the routine communications of the First U.S. Army Group. In one of the most bizarre tricks, the corpse of a drowned British civilian was dressed in the uniform of a headquarters officer and put into the sea off the coast of neutral Portugal, they broadcast faked radio messages from a disabled airplane, the fake officer had a mapcase with maps detailing the invasion plan for Calais, it was arranged that the body and maps were found by German agents in Portugal.
I believe there is no CAS No for oligonucleotide. However, here's the CAS No for DNA and RNA. Hope it will be helpful. Deoxyribonucleic Acid CAS No. 9007-49-2 Ribonucleic Acid… CAS No. 66082-38-0