0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Role of tris in extraction of DNA?
To give the solution buffering capacity.
What does baking soda do in DNA extraction?
Baking soda keeps the PH levels of the solution at an even level as the DNA is extracted.
What is the function of the alcohol in DNA extraction?
DNA is not soluble in isopropyl alcohol. It will precipitate out when you add this solvent. Once out of solution you can centrifuge it down and collect the pellet of DNA.
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
Role of STE solution in DNA extraction?
STE stabilize the lysosom's membrane stop the proteinase liberation
Function of phenol DNA extraction?
phenol helps to remove non polar proteins and lipids from the solution
What is the function of phenol in DNA extraction?
phenol helps to remove non polar proteins and lipids from the solution
Why saturated KCL in DNA extraction is used?
Saturated KCl precipitation is often used in DNA extraction for molluscan taxa. Molluscs produce a polysaccharide rich mucus that interferes with the reagents involved in DNA extraction. The KCl saturated solution is used right after the digestion step: about 1/4th of the volume of the digestion solution is added to the sample. Samples are then centrifugated at 14rpm for 15 minutes. The pellet formed will contain the polysaccharides and non digested tissue. The supernatant is extracted from the tube and used in the next steps of the DNA extraction.
What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
DNA extraction from bacteria?
DNA extraction from bacteria can be achieved in various ways. Yeast is the best resource to extract the DNA bacteria from using extreme rapid extraction method.
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
