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Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.

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Q: What is shake flask fermentation?
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Related questions

What are design aspects of shake flask fermentation?

Shake flasks, such as the Erlenmeyer flask, are designed to prevent splashing. They promote oxygen transfer and prevent vibration, allowing for successful fermentation.


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Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.


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UMMM....A bottle? i think it's called a decanter


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You get meth in about 40 minutes. Throw it all on one flask and let it sit for a while shake the flask it if starts to bubble up too much. Then when it calms down heat it to about 180f until it doesnt look like anything else is happening.


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