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Paper chromatography and TLC both use a solid stationary phase and liquid mobile phase. The only real difference is in the physical and chemical composition of the stationary phase. Paper chromatography uses a fibrous protein/polymer mesh (i.e. paper) as its stationary phase, TLC uses a compacted powder (usually silica or alumina) on a sealed backing.
Wiki User
∙ 16y ago
Wiki User
∙ 13y agoPaper chromatography and TLC both use a solid stationary phase and liquid mobile phase. The only real difference is in the physical and chemical composition of the stationary phase. Paper chromatography uses a fibrous protein/polymer mesh (i.e. paper) as its stationary phase, TLC uses a compacted powder (usually silica or alumina) on a sealed backing. TLC is more sensitive than PC
Wiki User
∙ 12y ago1.Rate of flow of the solvent.
2.Strength of absorption & distribution of substance of the mixture on the stationary phase.
Wiki User
∙ 11y agoThe two are inter-related in that we use TLC to determine what solvent or solvents will be effective in a large-scale column chromatographic separation.
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What is function of butanol in paper chromatography?
Butanol is used as a solvent in paper chromatography because it can dissolve a wide range of compounds. It helps to carry the sample and allow it to migrate up the paper. Butanol also helps in separating the components of the sample by interacting differently with different compounds.
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
Disadvantages of using paper chromatography?
TLC has following advantages over paper chromatography: greater speed greater sensitivity for many substances than paper Small sample requirement Usually sharper preparation Different kind of reagents can be applied without damaging the plate
Distinguish between TLC and hptlc?
TLC is Thin layer Chromatography while HPTLC is High Performance Thin Layer Chromatography HPTLC is Automotive ,It has Scanner,Injector,Developer -al automated and controleed generally by Software
What is a suitable solvent for paper chromatography?
In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent. Also, in some cases, DCM(dichloromethane is used as a solvent.
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
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What is function of butanol in paper chromatography?
Butanol is used as a solvent in paper chromatography because it can dissolve a wide range of compounds. It helps to carry the sample and allow it to migrate up the paper. Butanol also helps in separating the components of the sample by interacting differently with different compounds.
How do they do chromatography?
there are different types of chromatography e.g., thin layer chromatography, paper chromatography, gas chromatography, HPLC etc.TLC - THE PROCEDURE TO DO TLC IS GIVEN BELOWFirst of all a microscopic slide is taken and slurry made up of different components to make solid phase is poured on to the plate and then plate is put inside the hot air oven for the activation
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
Disadvantages of using paper chromatography?
TLC has following advantages over paper chromatography: greater speed greater sensitivity for many substances than paper Small sample requirement Usually sharper preparation Different kind of reagents can be applied without damaging the plate
What is the name of investigating colored substances?
Paper (Adsorption) Chromatography or Thin Layer Chromatography (TLC) or Solid-Liquid Chromatography (SLC)
Why can the pigment spot on chromatography paper not be submerged in solvent?
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
Distinguish between TLC and hptlc?
TLC is Thin layer Chromatography while HPTLC is High Performance Thin Layer Chromatography HPTLC is Automotive ,It has Scanner,Injector,Developer -al automated and controleed generally by Software
Which property of compounds is responsible for the separation in TLC?
The general Principle involved in tlc is similar to that of column chromatography i.e. adsorption chromatography. In the adsorption process, the solute competes with the solvent for the surface sites of the adsorbent. Depending on the distribution coefficients, the compounds are distributed on the surface of the adsorbent.
Which chromatographic process has minimal equipment and space requirements?
(TLC) Thin-Layer Chromatography
Where I can buy Silica Gel TLC Plates for Column Chromatography?
you can find these items in alibaba or sigmaaldrich website.
