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Why does catalase elute in a wider range of fractions than glucose oxidase does?
Wiki User
∙ 7y ago
Catalase degrades easily at room temperature b. Glucose oxidase is inactivated at low pH c. Glucose oxidase is only active at low pH d. Glucose oxidase has a higher molecular weight than catalase .
Wiki User
∙ 7y ago
Isabel Ibeson
B
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Which of the compounds elute fast in reverse phase chromatography?
chloroform
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
How can you further isolate pigments on a thin layer plate in TLC experiment?
Run the mixture on the TLC plate. Find the different colored pigments. Scrape each spot and elute the pigments.
What is void volume in hplc?
Total volume of mobile phase in a fully wet packed column- the space between the particles of the stationary phase plus the volume within the particles
How do you purify impure DNA after restriction digestion?
You can do a tedious phenol-chloroform extraction, or do it the easy way: QIAquick PCR purification kit where you bind the DNA to a column and elute it off in water or TE. You will lose some of your DNA though so keep this in mind.
Why do phosphate ions elute before sulfate ions in ion chromatography?
They can actually elute in either order. It depends on your mobile phase. In a weakly basic solution, the phosphate ions are found more as HPO4 (2-) rather than PO4 (3-) and thus will elute before sulfate which is larger and has the same charge. In a strongly basic mobile phase, the PO4 (3-) ion will dominate, and will elute after the sulfate ion
Do alkenes elute before alkanes in gas chromatography?
Usually, but not always. Depends on the bond position and polarity of the column. Alkenes are less likely to elute first on polar columns.
Which of the compounds elute fast in reverse phase chromatography?
chloroform
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What scrabble can be made from the letters eeuuttl?
Elute, Ettle, Tutee, Lute, Teel, Telt, Tutu, Eel, Ut, Te, Et
How can you further isolate pigments on a thin layer plate in TLC experiment?
Run the mixture on the TLC plate. Find the different colored pigments. Scrape each spot and elute the pigments.
What is void volume in hplc?
Total volume of mobile phase in a fully wet packed column- the space between the particles of the stationary phase plus the volume within the particles
How do you purify impure DNA after restriction digestion?
You can do a tedious phenol-chloroform extraction, or do it the easy way: QIAquick PCR purification kit where you bind the DNA to a column and elute it off in water or TE. You will lose some of your DNA though so keep this in mind.
Why should the amino acids spots not be submerged within the solvent?
I assume you are talking about thin-layer chromatography. If the spots are submerged in the solvent, they will dissolve into it and become so diluted that they will most likely be undetectable. Plus, they will elute as a band, not as a spot. Your solvent will also be contaminated.
Role of Ion suppressor in ion chromatography?
The suppressor reduces the background conductivity of the chemicals used to elute samples from the ion-exchange column. This improves the conductivity measurement of the ions being tested. check it http://www.dionex.com/en-us/products/accessories/suppressors/lp-73569.html
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
