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Q: Can Kliglers Iron Agar tell the difference between e coli and e aerogenes?
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Does enterobacter aerogenes produce red and white colonies in nutrient agar?

white colonies


What is the gram stain of enterobacter aerogenes?

Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures betwee 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures between 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Klebsiella is positive for urease production while Enterobacter is negative.


Does Enterobacter aerogenes produce gas in the Kligler Iron Agar test?

Yes. Enterobacter aerogenes ferments lactose and glucose as well as producing gas. It does not produce H2S. Occasionally the slant may revert to an alkaline reaction even though lactose has been fermented. Another lactose test should be formed, such as the reaction on MacConkey agar


Is enterobacter aerogenes lipase negative?

Yes it is. You can easily check it out yourself by using a tributyrine based agar. If Enterobacter aerogenes builds a lipase action enzyme, the surrounding of your colonies will be much clearer. If you have any other questions, I suggest you the Bergey's manual of systematic bacteriology.


Can enterobacter aerogenes grow on mannitol salt agar?

the bacteria dont ferment in MSA, there is no growth on the plat


What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?

Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.


What are the major differences between blood and chocolate agar?

In chocolate agar , blood is lysed by temperature, from that differnt matrials which are helpful to some organisms are release from RBCs.. and from blood agar cells are not lysed and some organisms can not take their nutrients from unlysed RBCs... This is a main difference....


What is the similarities between agar-agar and gelatin?

They're both used as thickeners in cooking


Difference between data structure and dbms?

deti chod...bhonsdi wale agar ans nhi pata hai to kahe web site khol k baitha hai..


Agar agar grain found in Germany?

Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores


What is the difference between nutrient agar culture media and MacConkey agar culture media?

nutrient agar is used generally for culturing any organism.But Muller hinton agar is specifically used for testing antibiotic sensitivity as it does not contain any inhibitory substances for the growh of the organism


Is there a microorganism that degrades Agar agar?

Yes! Paenibacillus spp. can degrade agar agar