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The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.
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β 16y ago
The Sakaguchi test is a chemical test used to detect the presence of guanidine compounds, such as arginine, in a sample. The test involves the reaction of guanidine groups with Ξ±-naphthol in the presence of sodium hypobromite to form a red-colored complex. This test is commonly used in biochemistry and organic chemistry to identify guanidine-containing compounds.
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β 9y agoThe Sakaguchi test is a chemical test that detects the presence of arginine in proteins. The guanidine in the arginine reacts with the 1-Naphthol in the reagent, forming a red-colored substance.
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What is the positive result for sakaguchi test?
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
Sakaguchi test for arginine?
The Sakaguchi test is a chemical test used to detect the presence of arginine in a sample. It involves the formation of a colored dye complex when arginine reacts with ninhydrin in an acid medium. The intensity of the color produced is proportional to the amount of arginine present in the sample.
Why should there be an order of addition of the reagents in the sakaguchi's test?
The order of addition in Sakaguchi's test is important to prevent false positives or precipitation of chemicals. Adding Ξ±-naphthol before sulfuric acid helps in the formation of a stable color complex with guanidine, avoiding unwanted reactions that could interfere with the test results.
What is wet test of chemistry?
A wet test is realized in solution.
What is the active ingredeint in sakaguchi reagent?
The active ingredient in Sakaguchi reagent is Ξ±-naphthol. It is used for the detection of arginine in biological samples by forming a red-colored precipitate in the presence of arginine.
How does Sakaguchi test works?
Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.
What is the positive result for sakaguchi test?
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
Sakaguchi test for arginine?
The Sakaguchi test is a chemical test used to detect the presence of arginine in a sample. It involves the formation of a colored dye complex when arginine reacts with ninhydrin in an acid medium. The intensity of the color produced is proportional to the amount of arginine present in the sample.
When did Kinichiro Sakaguchi die?
Kinichiro Sakaguchi died in 1994.
When was Ango Sakaguchi born?
Ango Sakaguchi was born in 1906.
When was Hiroyuki Sakaguchi born?
Hiroyuki Sakaguchi was born in 1965.
When was ShΕ«hei Sakaguchi born?
Shūhei Sakaguchi was born in 1977.
When was Chikara Sakaguchi born?
Chikara Sakaguchi was born in 1934.
When was Kinichiro Sakaguchi born?
Kinichiro Sakaguchi was born in 1897.
When was Alicja Sakaguchi born?
Alicja Sakaguchi was born in 1954.
How tall is Seiji Sakaguchi?
Seiji Sakaguchi is 6' 5".
How tall is Tak Sakaguchi?
Tak Sakaguchi is 5' 7".
