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Q: Does pH influence the gram stain reaction?
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What are 3 factors which can influence the activity of an enzyme?

temperature, PH and concentration of substrate (reaction).


Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?

8


What is the pH of a carbolfuchsin direct stain?

Acidic


What is the pH of buffer in the romanowsky stain?

6.8


How does the reaction when blood pH is low compare to the reaction when blood pH is high?

We need to see what reaction that you are referring is.


Does NaCl influence the pH in a buffer?

The water solution of sodium chloride is neutral; any influence on pH.


Could a negatively charged dye stain a bacteria?

The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.


Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?

lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7


What is a neutralzation reaction?

A neutralization reaction occurs between an acid and a base. As you may know, acids have a pH between 0 and 7, neutral substances have a pH of 7, and bases have a pH between 7 and 14. In a neutralization reaction, the pH's of the reactants are such the product(s) has/ have a pH of 7.


What is the pH of dibutyl phthalate?

Since it is almost insoluble in water (< 13 mg/L) there is no pH influence on the normal water pH = 7. Besides: concentration (not mentioned) is of big influence on pH!


What is the optimum PH of lactase catalyzed reaction?

The optimum pH of lactase is pH = 6.5.


What is the influence of the pH on the gram reaction in gram staining?

In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.