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How can a mutation that alters a recognition site be detected by gel electrophoresis?
Wiki User
∙ 15y ago
First, DNA that is mutated and unmutated must be cut with the same restriction enzyme. When these two strains of DNA are run through gel electrophoresis side by side, the mutated DNA will have fewer bands and at least one that does not move as far as the normal DNA. This is because the the restriction enzyme would not cut at the mutated recognition site. The difference in bands in the agarose gel will easily be detected.
Wiki User
∙ 15y ago
Wiki User
∙ 12y agoA mutation in DNA can alter the sequence where a restriction enzyme cleaves the dna prior to running the gell. This means that two different band patterens would appear when running One mutated and one native sequence of dna.
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How do chromosome mutations affect an individual?
Chromosome mutations can result in changes in the number of chromosomes in a cell or changes in the structure of a chromosome. Unlike a gene mutation which alters a single gene or larger segment of DNA on a chromosome, chromosome mutations change and impact the entire chromosome.
How does DNA molecules show constancy and variation from one species to the next?
DNA replication follows the semi-conservative model, base pairing and complentary RNA are formed, in a '5 to '3 direction, so the new strands will have old and new material but look the same as the original strand. variation occurs due to the genetic mutation that alters the base-pairing which is sometimes due to a problematic polymerase"2 enzyme, and this mutation will alter the genes structure permanently.
What change occurs that alters the appearance of matter?
physical change
You can touch boiling water on the mountain tops why?
Because the atmospheric pressure upon the mountain top alters the amount of kinetic energy (movement of the particles) or in plain English alters the amount of heat the water gives off.
The most commonly used hallucinogen which alters the effect of serotonin is called what?
LSD or acid
How can a mutation that alters a recognition site be detected in gel electrophoresis?
The point mutation has to result in either the removal of a restriction site of the restriction enzymes or the formation of a new one, such that the bands of mutated DNA that form after performing gel electrophoresis are different from the normal one. So a difference in banding patterns would mean that there is a point mutation.
How does a nonsense suppressor mutation prevent amber mutants from terminating their polypeptides prematurely?
The mutation alters a tRNA so that it reads the amber codon and inserts an amino acid.
What One example of a mutation that would not affect an organisms phenotype?
A Missense Mutation.It is a point mutation where the mutations alters the base sequence without changing the resulting function of the subsequent protein. This is usually when the mutation does not change the amino acid which the codon codes for.
Are super heros like Superman real?
yes if they create a mutation process that alters the genetic transformation in the body cells at birth, it could possibly happen.
A mutation that alters a single amino acid within a protein can alter?
the primary, secondary, and tertiary level of a protein structure because once an amino acid is effected by a mutation in a single amino acid it ruins the entire protein on all levels
What is a synonymous polymorphism?
A synonymous polymorphism is where a mutation alters the base in the DNA sequence but doesn't alter the amino acid encoded (due to the redundancy of the genetic code). Typically, for it to be classified as a polymorphism (as opposed to a mutation) it must occur in greater than 1% of the population.
List two types of gene mutations?
Two types of gene mutations are chromosomal and point mutation. Chromosomal mutation alters a cell's chromosomes, while point mutation replaces single-base pair nucleotides with other nucleotides found in RNA or DNA.
What kind of process alters molecular composition?
A chemical reaction alters molecular composition.
Comment dit-on alters en francais?
it means 'How do you say in French alters'.-mr.schizer
How many amino acids are affected from point mutation?
A point mutation can affect the protein in a different ways. If the point change causes a silent mutation then it doesnt affect at all. When the point nucleotide change make a different amino acid, then it may alters the function of protein. If it make to forma a stop codon (TAA, TAG, TGA) then the protein synthesis stops at the point where it is changed.
Can you give an example of a sentence using the word alters?
The new fuel mixture alters the engine performance
How do chromosome mutations affect an individual?
Chromosome mutations can result in changes in the number of chromosomes in a cell or changes in the structure of a chromosome. Unlike a gene mutation which alters a single gene or larger segment of DNA on a chromosome, chromosome mutations change and impact the entire chromosome.
