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How do activator proteins help RNA polymerase bind to a strand of DNA?
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What is main function of DNA polymerase?
The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
What are the proteins that bind to DNA and turn on operons by making it easier for RNA polymerase to bind to a promoter called?
transcription factor
What are a group of proteins that bind to the promoter or enhancer sequences to facilitate the binding of RNA polymerase to the promoter?
transcription factors
What do single stranded binding proteins do?
When double stranded DNA is unwound into single stranded DNA, single-strand binding proteins bind to each single stranded DNA strand and prevent the two strands from reattaching to each other, allowing DNA replication to continue.
What name is given to the group of proteins that bind to the unzipped DNA to keep the DNA bases from re-bonding once they have unzipped?
The mRNA strand!
What is main function of DNA polymerase?
The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
Proteins that bind to DNA and turn on operons by making it easier for RNA polymerase to bind to a promoter are called?
Activators
What are the proteins that bind to DNA and turn on operons by making it easier for RNA polymerase to bind to a promoter called?
transcription factor
What are a group of proteins that bind to the promoter or enhancer sequences to facilitate the binding of RNA polymerase to the promoter?
transcription factors
What do the template strands of DNA always begin with?
DNA is made of of two complimentary strands, the coding strand and the template strand. When DNA is transcribed (made into messenger RNA which can be converted by ribosomes into proteins) the DNA splits open and free nucleotide bases bind to the template strand. DNA is made of T/C/G/A and RNA is made of U/C/G/A nucleotide bases. G and C bind (they are said to be 'complimentary') A and T bind and in RNA U and A bind (so U replaces T.) The newly formed RNA strand (made on the template stand of DNA) is 'complimentary' to the template but the same as the coding strand of DNA. Hence the template is used to produce RNA which is a copy of the coding strand. Either strand of DNA can act as the template/coding strand. Hope that is a little bit helpful!
What do single stranded binding proteins do?
When double stranded DNA is unwound into single stranded DNA, single-strand binding proteins bind to each single stranded DNA strand and prevent the two strands from reattaching to each other, allowing DNA replication to continue.
Role of additives in PCR?
Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O
What does bacterial rna polymerase bind to?
promoter
What happens to RNA polymerase ll after it has completed transcription of a gene?
RNA polymerase matches a base on the DNA to a RNA nucleotide(by complementary base pair binding) and then adds that new nucleotide to the elongating mRNA strand. A messenger RNA strand is released from RNA polymerase. This strand is a complementary copy of the DNA message and can now move to the process of translation
What name is given to the group of proteins that bind to the unzipped DNA to keep the DNA bases from re-bonding once they have unzipped?
The mRNA strand!
Where does ssb protein bind on lagging strand or on leading strand?
ssb protein bind to the lagging strand as leading strand is invovled in dna replication and lagging strand is invovled in okazaki fragment formation
What is the region of DNA to which RNA polymerase bind?
promoter
