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When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?
DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.
What is a technique for sorting DNA fragments by length?
it is called " electrophoresis"
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Are restriction enzymes used in gel electrophoresis?
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?
DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.
What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?
They would be the same since Dolly is clone.
Why does the the florescense appear in the chaneels only n not in the form of moving bands While performing Gel electrophoresis For DNA detection?
Only the bands should show fluorescence, you must be doing the technique wrong..
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
What do DNA bands represent in the agarose gel electrophoresis?
Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.
