What you mean by the performance of Biuret Test? This method just can tell us that if there is any protein contents but it can't give any exact figure of the type of protein. The Beer's law is obeyed to at least 10 g per dl. So actually what your question point of? What you mean by the performance of Biuret Test? This method just can tell us that if there is any protein contents but it can't give any exact figure of the type of protein. The Beer's law is obeyed to at least 10 g per dl. So actually what your question point of?
Proteins are made of peptide bonds which is the target of reaction for biuret test. It's not truly specific for proteins; biuret itself also gives a positive result (hence the name of the test). However, proteins are fairly common and biuret (and similar peptide-containing compounds) are relatively rare.
Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.
Benedict's solution is used to test for simple sugars, and Biuret solution is used to test for proteins.
Copper sulphate gives biuret reagent it's blue colour.
The basics of Biuret Protein Assay method include few interfering agents, one of which is ammonium salts. There are fewer deviations than ultraviolet absorption methods, but Biuret method consumes much more materials.
The biuret solution used in the biuret test for peptide bonds is blue in the absence of peptide bonds or biuret which also results in a positive result. A positive result for peptide bonds or biuret is apparent as the biuret solution turns purple/violet in color.
Buiret Reagent
Biuret solution
protein
food that has protein like milk will cause the Biuret solution to turn purple.
biuret reagent
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
Found this place in my own personal quest for some. There might be better. http://www.sciencecompany.com/sci-exper/food_chemistry.htm#Biuret%20Reagent
It cuts/breaks the peptide bonds.
process that release energy by breaking down food molecules in the presence of oxygen
Both cows milk and water-buffalo's milk produce a violet colour when tested with biuret solution because they both contain protein.
Alkaline levels are essential for the solution to change colour. Without alkaline levels, the colour of the solution would not be detectable.