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The basics of Biuret Protein Assay method include few interfering agents, one of which is ammonium salts. There are fewer deviations than ultraviolet absorption methods, but Biuret method consumes much more materials.

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9y ago
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13y ago

The biuret test is a chemical test used for detecting the presence of peptide bonds. In the presence of peptides, a copper(II) ion forms a violet-colored complex in an alkaline solution.

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Q: What are the basics of biuret method?
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Related questions

Name the important chemical method available to determine total protein in the body fluids?

Biuret test


What is biuret reagent used for?

Biuret reagent detect peptide bonds.


Does potato and biuret turns violet?

onion and biuret turns violet?


What is the color of biruet solution?

The biuret solution used in the biuret test for peptide bonds is blue in the absence of peptide bonds or biuret which also results in a positive result. A positive result for peptide bonds or biuret is apparent as the biuret solution turns purple/violet in color.


What is the reagent used to test proteins?

Buiret Reagent


Caseine in biuret test?

Casein form a purple compound in the biuret test.


How can I find out whether a unknown mixture has protein in it?

The most common method is the biuret test: in the presence of a copper(II) solution and NaOH the sample become violet.


How do you prepare biuret solution?

What you mean by the performance of Biuret Test? This method just can tell us that if there is any protein contents but it can't give any exact figure of the type of protein. The Beer's law is obeyed to at least 10 g per dl. So actually what your question point of? What you mean by the performance of Biuret Test? This method just can tell us that if there is any protein contents but it can't give any exact figure of the type of protein. The Beer's law is obeyed to at least 10 g per dl. So actually what your question point of?


Will glutamic acid give a positive biuret test?

Amino acids doesn't react with the biuret reagent.


What is an indicator for Albumin?

Biuret


What might Biuret reagent test in a practical real life situation?

Biuret reagent test in a practical situation tests for lipids. Biuret is used in food agents for protein amino acid chains.


Is biuret test suitable to test the presence of proteins in the urine?

Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.