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Pcr
I think you should increase the annealing temperature and also reduce the PCR cycles.
yes for example 2D gel electrophoresis
gel
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
Pcr
Gel Electrophoresis
I think you should increase the annealing temperature and also reduce the PCR cycles.
what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
yes for example 2D gel electrophoresis
Gel electrophoresis
PCR or polymerase chain reaction. :: Apex
gel
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
PCR products produce million copies of your gene of interest. After PCR, we usually resolve them on the agarose gel to visualize the amplified DNA using EtBr stain under UV. The main purpose is, it make sure your gene is really amplified and the length it run is corresponding to the right size of your gene of interest and purify it from other template DNA and other unspecifically amplified DNA products by extracting from the gel.